tetano
Editor, Senior Moderator
J Virol Methods. 2013 Jun 10. pii: S0166-0934(13)00219-X. doi: 10.1016/j.jviromet.2013.06.006. [Epub ahead of print]
Evaluation of the Pan Influenza Detection Kit Utilizing the PLEX-ID and Influenza Samples from the 2011 Respiratory Season.
Murillo L, Hardick J, Jeng K, Gaydos CA.
Source
The Johns Hopkins University School of Medicine, Division of Infectious Diseases, 855 North Wolfe Street, Rangos Building, Room 530, Baltimore, MD, 21205 United States. Electronic address: lmurill2@jhmi.edu.
Abstract
A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N=75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For Influenza viruses identified as H1N1 pdm 09, Influenza A virus A/NEW YORK/15/2009 (H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm 09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm 09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).
Copyright ? 2013. Published by Elsevier B.V.
PMID:
23764420
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23764420
Evaluation of the Pan Influenza Detection Kit Utilizing the PLEX-ID and Influenza Samples from the 2011 Respiratory Season.
Murillo L, Hardick J, Jeng K, Gaydos CA.
Source
The Johns Hopkins University School of Medicine, Division of Infectious Diseases, 855 North Wolfe Street, Rangos Building, Room 530, Baltimore, MD, 21205 United States. Electronic address: lmurill2@jhmi.edu.
Abstract
A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N=75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For Influenza viruses identified as H1N1 pdm 09, Influenza A virus A/NEW YORK/15/2009 (H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm 09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm 09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).
Copyright ? 2013. Published by Elsevier B.V.
PMID:
23764420
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23764420