tetano
Editor, Senior Moderator
J Med Microbiol. 2016 Mar 11. doi: 10.1099/jmm.0.000249. [Epub ahead of print]
[h=1]Evaluation of the Alere-i Influenza A&B assay for Rapid Identification of Influenza A and Influenza B Viruses.[/h] Chiarella FC[SUP]1[/SUP], Culebras E[SUP]2[/SUP], Fuentes-Ferrer ME[SUP]3[/SUP], Picazo JJ[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The Alere-i Influenza A&B assay is a novel isothermal nucleic acid amplification assay capable of detecting and differentiating influenza A and B viruses in approximately 15 minutes with minimal hands-on time. This study was conducted in order to study the performance of Alere-i Influenza A&B assay compared to molecular techniques, considered as gold standard methods to evaluate the results. A total of 119 nasopharyngeal swabs collected from inpatients with influenza-like illness were included in the study using both archived and prospectively collected samples from adults and children. Prospectively collected samples were also compared to Alere BinaxNOW? Influenza A & B Card. The overall sensitivity for detection of Influenza A and B viruses compared to those of molecular techniques were 65.96% and 53.33% respectively, while the specificity were 98.51% and 95.96%. Compared to Alere BinaxNOW? Influenza A & B Card, the Alere-i assay is considerably more sensitive for detection of influenza A and B viruses, although both tests demonstrated excellent specificity for diagnosis of Influenza viruses.
PMID: 26967368 [PubMed - as supplied by publisher]
[h=1]Evaluation of the Alere-i Influenza A&B assay for Rapid Identification of Influenza A and Influenza B Viruses.[/h] Chiarella FC[SUP]1[/SUP], Culebras E[SUP]2[/SUP], Fuentes-Ferrer ME[SUP]3[/SUP], Picazo JJ[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The Alere-i Influenza A&B assay is a novel isothermal nucleic acid amplification assay capable of detecting and differentiating influenza A and B viruses in approximately 15 minutes with minimal hands-on time. This study was conducted in order to study the performance of Alere-i Influenza A&B assay compared to molecular techniques, considered as gold standard methods to evaluate the results. A total of 119 nasopharyngeal swabs collected from inpatients with influenza-like illness were included in the study using both archived and prospectively collected samples from adults and children. Prospectively collected samples were also compared to Alere BinaxNOW? Influenza A & B Card. The overall sensitivity for detection of Influenza A and B viruses compared to those of molecular techniques were 65.96% and 53.33% respectively, while the specificity were 98.51% and 95.96%. Compared to Alere BinaxNOW? Influenza A & B Card, the Alere-i assay is considerably more sensitive for detection of influenza A and B viruses, although both tests demonstrated excellent specificity for diagnosis of Influenza viruses.
PMID: 26967368 [PubMed - as supplied by publisher]