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Evaluation of swabs, transport media and specimen transport conditions for optimal detection of viruses by PCR

tetano

Editor, Senior Moderator
J Clin Microbiol. 2011 Dec 28. [Epub ahead of print]
Evaluation of swabs, transport media and specimen transport conditions for optimal detection of viruses by PCR.
Druce J, Garcia K, Tran T, Papadakis G, Birch C.
Source

Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn Street North Melbourne, 3051, Victoria, Australia.
Abstract

Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus and adenovirus. Each was detected at ambient temperature (22?C) and 4?C through 7 days. Detection of influenza on dry or saline swabs is important because of its capacity to cause outbreaks involving high case numbers.

PMID:
22205810
[PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/22205810
 
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