tetano
Editor, Senior Moderator
J Vet Diagn Invest. 2018 Sep 28:1040638718803999. doi: 10.1177/1040638718803999. [Epub ahead of print]
[h=1]Evaluation of nonwoven fabrics for nasal wipe sampling for influenza A virus in swine.[/h] Nelson SW[SUP]1[/SUP], Hammons CT[SUP]1[/SUP], Bliss NT[SUP]1[/SUP], Lauterbach SE[SUP]1[/SUP], Zentkovich MM[SUP]1[/SUP], Lorbach JN[SUP]1[/SUP], Nolting JM[SUP]1[/SUP], Bowman AS[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus (IAV) is a zoonotic pathogen threatening animal and public health; therefore, detection and monitoring of IAV in animal populations are critical components of a surveillance program. Swine are important hosts of IAV, wherein the virus can undergo rapid evolution. Several methods (i.e., nasal swabs, nasal wipes, and oral fluids) have been used to collect samples from swine for IAV surveillance. We utilized nasal wipes made from cotton gauze and multiple, polyester or mixed polyester fabrics to compare performance in the molecular detection and isolation of IAV. In vitro experiments revealed that no polyester or mixed polyester fabric was superior to cotton gauze for molecular IAV detection; however, 3 polyester or mixed polyester fabrics yielded significantly more viable IAV than cotton. In a field trial, both cotton gauze and the polyester or mixed polyester fabric yielded similar proportions of IAV isolates from swine. The results indicate that cotton gauze remains a practical and useful material for swine nasal wipes.
[h=4]KEYWORDS:[/h] Animal population groups; influenza A virus; nose; polyesters; public health; swine; textiles
PMID: 30264670 DOI: 10.1177/1040638718803999
[h=1]Evaluation of nonwoven fabrics for nasal wipe sampling for influenza A virus in swine.[/h] Nelson SW[SUP]1[/SUP], Hammons CT[SUP]1[/SUP], Bliss NT[SUP]1[/SUP], Lauterbach SE[SUP]1[/SUP], Zentkovich MM[SUP]1[/SUP], Lorbach JN[SUP]1[/SUP], Nolting JM[SUP]1[/SUP], Bowman AS[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus (IAV) is a zoonotic pathogen threatening animal and public health; therefore, detection and monitoring of IAV in animal populations are critical components of a surveillance program. Swine are important hosts of IAV, wherein the virus can undergo rapid evolution. Several methods (i.e., nasal swabs, nasal wipes, and oral fluids) have been used to collect samples from swine for IAV surveillance. We utilized nasal wipes made from cotton gauze and multiple, polyester or mixed polyester fabrics to compare performance in the molecular detection and isolation of IAV. In vitro experiments revealed that no polyester or mixed polyester fabric was superior to cotton gauze for molecular IAV detection; however, 3 polyester or mixed polyester fabrics yielded significantly more viable IAV than cotton. In a field trial, both cotton gauze and the polyester or mixed polyester fabric yielded similar proportions of IAV isolates from swine. The results indicate that cotton gauze remains a practical and useful material for swine nasal wipes.
[h=4]KEYWORDS:[/h] Animal population groups; influenza A virus; nose; polyesters; public health; swine; textiles
PMID: 30264670 DOI: 10.1177/1040638718803999