Euro Surveill. Highly heterogeneous temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates, northern France

[Giuseppe]

[Source: Eurosurveillance, full text: (LINK). Abstract, edited.]

Eurosurveillance, Volume 16, Issue 43, 27 October 2011

Research articles


Highly heterogeneous temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates, northern France


I Pelletier<SUP>1</SUP><SUP>,2</SUP>, D Rousset<SUP>3</SUP><SUP>,4</SUP><SUP>,5</SUP><SUP>,6</SUP>, V Enouf<SUP>3</SUP><SUP>,4</SUP><SUP>,5</SUP><SUP>,6</SUP>, GROG<SUP>7</SUP>, F Colb?re-Garapin<SUP>1</SUP><SUP>,2</SUP>, S van der Werf<SUP>3</SUP><SUP>,4</SUP><SUP>,5</SUP><SUP>,6</SUP>, N Naffakh ()<SUP>3</SUP><SUP>,4</SUP><SUP>,5</SUP>

1. Institut Pasteur, Unit? de Biologie des Virus Ent?riques, D?partement de Virologie, Paris, France
2. INSERM U994 (French National Institute of Health and Medical Research) Paris, France
3. Institut Pasteur, Unit? de G?n?tique Mol?culaire des virus ? ARN, D?partement de Virologie, Paris, France
4. CNRS URA3015 (French National Centre for Scientific Research), Paris, France
5. Universit? Paris Diderot, Sorbonne Paris Cit?, Unit? de G?n?tique Mol?culaire des virus ? ARN, Paris, France
6. Institut Pasteur, Centre National de R?f?rence des virus influenzae (R?gion Nord), Paris, France
7. Groupes R?gionaux d'Observation de la Grippe, Open Rome, Paris, France

<HR>
Citation style for this article: Pelletier I, Rousset D, Enouf V, GROG, Colb?re-Garapin F, van der Werf S, Naffakh N. Highly heterogeneous temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates, northern France. Euro Surveill. 2011;16(43)ii=19999. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19999
Date of submission: 19 July 2011 <HR>We assayed the temperature sensitivity of 2009 pandemic influenza A(H1N1) viral isolates (n=23) and seasonal influenza A(H1N1) viruses (n=18) isolated in northern France in 2007/08 and 2008/09. All isolates replicated with a similar efficiency at 34 ?C and 37 ?C, and with a lower efficiency at 40 ?C. The pandemic viral isolates showed a stronger heterogeneity in their ability to grow at the highest temperature, as compared with the seasonal isolates. No statistically significant difference in temperature sensitivity was observed between the pandemic viral isolates from severe and mild cases of influenza. Our data point to the impact of temperature sensitivity on the genetic evolution and diversification of the pandemic influenza A(H1N1) virus since its introduction into the human population in April 2009, and call for close surveillance of this phenotypic marker related to host and tissue tropism.
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