tetano
Editor, Senior Moderator
Yao Xue Xue Bao. 2012 Sep;47(9):1159-63.
[Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase].
[Article in Chinese]
Wang Z, Wang X, He Z, Liu ZL, Wei XL, Yin X, Zhou JM, Li XY, Zhang ZZ, Cen S.
Source
Department of lmmunology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Abstract
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
PMID:
23227545
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23227545
[Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase].
[Article in Chinese]
Wang Z, Wang X, He Z, Liu ZL, Wei XL, Yin X, Zhou JM, Li XY, Zhang ZZ, Cen S.
Source
Department of lmmunology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Abstract
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
PMID:
23227545
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23227545