tetano
Editor, Senior Moderator
J Immunol Methods. 2014 Apr 28. pii: S0022-1759(14)00122-7. doi: 10.1016/j.jim.2014.04.005. [Epub ahead of print]
Establishment and Maintenance of a PBMC Repository for Functional Cellular Studies in Support of Clinical Vaccine Trials.
Sambor A1, Garcia A2, Berrong M3, Pickeral J3, Brown S4, Rountree W2, Sanchez A2, Pollara J3, Frahm N5, Keinonen S2, Kijak GH6, Roederer M7, Levine G1, Patricia D'Souza M8, Jaimes M9, Koup R7, Denny T2, Cox J10, Ferrari G11.
Author information
Abstract
A large repository of cryopreserved peripheral blood mononuclear cells (PBMC) samples was created to provide laboratories testing the specimens from Human Immunodeficiency Virus-1 (HIV-1) vaccine clinical trials the material for assay development, optimization, and validation. One hundred thirty-one PBMC samples were collected using leukapheresis procedure between 2007 and 2013 by the Comprehensive T cell-Vaccine Immune Monitoring Consortium core repository. The donors included 83 Human Immunodeficiency Virus-1 (HIV-1) seronegative and 32 HIV-1 seropositive subjects. The samples were extensively characterized for the ability of T cell subsets to respond to recall viral antigens including Cytomegalovirus, Epstein-Barr virus, Influenza virus, and HIV-1 using Interferon-gamma (IFN-γ) Enzyme Linked ImmunoSpot (ELISpot) and IFN-γ/Interleukin 2 (IL-2) Intracellular Cytokine Staining (ICS) assays. A subset of samples was evaluated over time to determine the integrity of the cryopreserved samples in relation to recovery, viability, and functionality. The principal results of our study demonstrate that viable and functional cells were consistently recovered from the cryopreserved samples. Therefore, we determined that this repository of large size cryopreserved cellular samples constitutes an unique resource for laboratories who are involved in optimization and validation of assays to evaluate T, B, and NK cellular functions in the context of clinical trials.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Cryopreservation, Peripheral Blood Mononuclear Cells, Repository
PMID:
24787274
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24787274
Establishment and Maintenance of a PBMC Repository for Functional Cellular Studies in Support of Clinical Vaccine Trials.
Sambor A1, Garcia A2, Berrong M3, Pickeral J3, Brown S4, Rountree W2, Sanchez A2, Pollara J3, Frahm N5, Keinonen S2, Kijak GH6, Roederer M7, Levine G1, Patricia D'Souza M8, Jaimes M9, Koup R7, Denny T2, Cox J10, Ferrari G11.
Author information
Abstract
A large repository of cryopreserved peripheral blood mononuclear cells (PBMC) samples was created to provide laboratories testing the specimens from Human Immunodeficiency Virus-1 (HIV-1) vaccine clinical trials the material for assay development, optimization, and validation. One hundred thirty-one PBMC samples were collected using leukapheresis procedure between 2007 and 2013 by the Comprehensive T cell-Vaccine Immune Monitoring Consortium core repository. The donors included 83 Human Immunodeficiency Virus-1 (HIV-1) seronegative and 32 HIV-1 seropositive subjects. The samples were extensively characterized for the ability of T cell subsets to respond to recall viral antigens including Cytomegalovirus, Epstein-Barr virus, Influenza virus, and HIV-1 using Interferon-gamma (IFN-γ) Enzyme Linked ImmunoSpot (ELISpot) and IFN-γ/Interleukin 2 (IL-2) Intracellular Cytokine Staining (ICS) assays. A subset of samples was evaluated over time to determine the integrity of the cryopreserved samples in relation to recovery, viability, and functionality. The principal results of our study demonstrate that viable and functional cells were consistently recovered from the cryopreserved samples. Therefore, we determined that this repository of large size cryopreserved cellular samples constitutes an unique resource for laboratories who are involved in optimization and validation of assays to evaluate T, B, and NK cellular functions in the context of clinical trials.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Cryopreservation, Peripheral Blood Mononuclear Cells, Repository
PMID:
24787274
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24787274