tetano
Editor, Senior Moderator
Clin Infect Dis. 2010 May 19. [Epub ahead of print]
Enhanced Diagnosis of Pandemic (H1N1) 2009 Influenza Infection Using Molecular and Serological Testing in Intensive Care Unit Patients with Suspected Influenza.
Iwasenko JM, Cretikos M, Paterson DL, Gibb R, Webb SA, Smith DW, Blyth CC, Dwyer DE, Shi JQ, Robertson P, Rawlinson WD.
Department of Microbiology, South Eastern Area Laboratory Services, Prince of Wales Hospital, Randwick, 2New South Wales Department of Health, 3School of Public Health, University of Sydney, Sydney, 4Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, and 5School of Biotechnology and Biomolecular Sciences, Faculty of Science, and School of Medical Sciences, Faculty of Medicine, University of New South Wales, Kensington, New South Wales; 6University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital Campus, and 7Microbiology Central Laboratory, Pathology Queensland, Brisbane, Queensland; 8Intensive Care Unit, Royal Perth Hospital, Perth, 9PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Nedlands, and 10School of Biomedical and Chemical Sciences, Faculty of Life and Physical Sciences, and School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands, Western Australia, Australia.
Abstract
During the 2009 outbreak of pandemic (H1N1) 2009 influenza (pH1N1) in Australia, acute and convalescent serum specimens were collected from 33 patients with severe respiratory disease admitted to intensive care units. Using hemagglutination inhibition of pH1N1, 29 paired serum samples showed significant increases in specific antibody titers. Of these 29 patients, 18 had pH1N1 RNA detected by routine nucleic acid testing. These results indicate that up to one-third of pH1N1 cases may not have laboratory confirmation of infection unless serological testing is included for suspected cases.
PMID: 20482372 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20482372
Enhanced Diagnosis of Pandemic (H1N1) 2009 Influenza Infection Using Molecular and Serological Testing in Intensive Care Unit Patients with Suspected Influenza.
Iwasenko JM, Cretikos M, Paterson DL, Gibb R, Webb SA, Smith DW, Blyth CC, Dwyer DE, Shi JQ, Robertson P, Rawlinson WD.
Department of Microbiology, South Eastern Area Laboratory Services, Prince of Wales Hospital, Randwick, 2New South Wales Department of Health, 3School of Public Health, University of Sydney, Sydney, 4Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, and 5School of Biotechnology and Biomolecular Sciences, Faculty of Science, and School of Medical Sciences, Faculty of Medicine, University of New South Wales, Kensington, New South Wales; 6University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital Campus, and 7Microbiology Central Laboratory, Pathology Queensland, Brisbane, Queensland; 8Intensive Care Unit, Royal Perth Hospital, Perth, 9PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Nedlands, and 10School of Biomedical and Chemical Sciences, Faculty of Life and Physical Sciences, and School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands, Western Australia, Australia.
Abstract
During the 2009 outbreak of pandemic (H1N1) 2009 influenza (pH1N1) in Australia, acute and convalescent serum specimens were collected from 33 patients with severe respiratory disease admitted to intensive care units. Using hemagglutination inhibition of pH1N1, 29 paired serum samples showed significant increases in specific antibody titers. Of these 29 patients, 18 had pH1N1 RNA detected by routine nucleic acid testing. These results indicate that up to one-third of pH1N1 cases may not have laboratory confirmation of infection unless serological testing is included for suspected cases.
PMID: 20482372 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20482372