tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2021 May;27(5):1362-1370.
doi: 10.3201/eid2705.204055.
Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands
Shan Zhao, Nancy Schuurman, Wentao Li, Chunyan Wang, Lidwien A M Smit, Els M Broens, Jaap A Wagenaar, Frank J M van Kuppeveld, Berend-Jan Bosch, Herman Egberink
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats and dogs, we developed and validated a set of serologic assays, including ELISA and virus neutralization. Evaluation of samples from animals before they acquired coronavirus disease and samples from cats roaming SARS-CoV-2-positive mink farms confirmed the suitability of these assays for specific antibody detection. Furthermore, our findings exclude SARS-CoV-2 nucleocapsid protein as an antigen for serologic screening of cat and dog samples. We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.
Keywords: COVID-19; ELISA; SARS-CoV-2; cats; coronavirus disease; coronaviruses; dogs; nucleocapsid protein; receptor-binding domain; respiratory infections; serologic screening; severe acute respiratory syndrome coronavirus 2; the Netherlands; virus neutralization; viruses; zoonoses.
. 2021 May;27(5):1362-1370.
doi: 10.3201/eid2705.204055.
Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands
Shan Zhao, Nancy Schuurman, Wentao Li, Chunyan Wang, Lidwien A M Smit, Els M Broens, Jaap A Wagenaar, Frank J M van Kuppeveld, Berend-Jan Bosch, Herman Egberink
- PMID: 33900184
- DOI: 10.3201/eid2705.204055
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats and dogs, we developed and validated a set of serologic assays, including ELISA and virus neutralization. Evaluation of samples from animals before they acquired coronavirus disease and samples from cats roaming SARS-CoV-2-positive mink farms confirmed the suitability of these assays for specific antibody detection. Furthermore, our findings exclude SARS-CoV-2 nucleocapsid protein as an antigen for serologic screening of cat and dog samples. We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.
Keywords: COVID-19; ELISA; SARS-CoV-2; cats; coronavirus disease; coronaviruses; dogs; nucleocapsid protein; receptor-binding domain; respiratory infections; serologic screening; severe acute respiratory syndrome coronavirus 2; the Netherlands; virus neutralization; viruses; zoonoses.