tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2022 Feb 7;28(4).
doi: 10.3201/eid2804.220146. Online ahead of print.
Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
Adriana Heguy, Dacia Dimartino, Christian Marier, Paul Zappile, Emily Guzman, Ralf Duerr, Guiqing Wang, Jonathan Plitnick, Alexis Russell, Daryl M Lamson, Kirsten St George
Abstract
Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; amplification artifact; coronavirus disease; genomics; respiratory infections; sequence analysis; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.
. 2022 Feb 7;28(4).
doi: 10.3201/eid2804.220146. Online ahead of print.
Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
Adriana Heguy, Dacia Dimartino, Christian Marier, Paul Zappile, Emily Guzman, Ralf Duerr, Guiqing Wang, Jonathan Plitnick, Alexis Russell, Daryl M Lamson, Kirsten St George
- PMID: 35130474
- DOI: 10.3201/eid2804.220146
Abstract
Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; amplification artifact; coronavirus disease; genomics; respiratory infections; sequence analysis; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.