tetano
Editor, Senior Moderator
ACS Nano. 2011 Nov 25. [Epub ahead of print]
Effectively and Efficiently Dissecting the Infection of Influenza Virus by Quantum Dot-Based Single-Particle Tracking.
Liu SL, Zhang ZL, Tian ZQ, Zhao HS, Liu H, Sun EZ, Xiao GF, Zhang W, Wang HZ, Pang DW.
Abstract
Exploring the virus infection mechanisms is significant for defending against virus infection and providing basis for studying endocytosis mechanisms. Single-particle tracking technique is a powerful tool to monitor virus infection in real time for obtaining dynamic information. In this study, we reported a quantum dots-based single-particle tracking technique to efficiently and globally research the virus infection behaviors in individual cells. It was observed that many influenza viruses were moving rapidly, converging to the microtubule organizing center (MTOC), interacting with acidic endosomes and finally entering the target endosomes for genome release, which provides a vivid portrayal of the five-stage virus infection process. This report settles a long-pending question of how viruses move and interact with acidic endosomes before genome release in the perinuclear region, and also finds that influenza virus infection is likely a 'MTOC rescue' model for genome release. The systemic technique developed in this report is expected to be widely used for studying the mechanisms of virus infection and uncovering the secrets of endocytosis.
PMID:
22117089
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22117089
Effectively and Efficiently Dissecting the Infection of Influenza Virus by Quantum Dot-Based Single-Particle Tracking.
Liu SL, Zhang ZL, Tian ZQ, Zhao HS, Liu H, Sun EZ, Xiao GF, Zhang W, Wang HZ, Pang DW.
Abstract
Exploring the virus infection mechanisms is significant for defending against virus infection and providing basis for studying endocytosis mechanisms. Single-particle tracking technique is a powerful tool to monitor virus infection in real time for obtaining dynamic information. In this study, we reported a quantum dots-based single-particle tracking technique to efficiently and globally research the virus infection behaviors in individual cells. It was observed that many influenza viruses were moving rapidly, converging to the microtubule organizing center (MTOC), interacting with acidic endosomes and finally entering the target endosomes for genome release, which provides a vivid portrayal of the five-stage virus infection process. This report settles a long-pending question of how viruses move and interact with acidic endosomes before genome release in the perinuclear region, and also finds that influenza virus infection is likely a 'MTOC rescue' model for genome release. The systemic technique developed in this report is expected to be widely used for studying the mechanisms of virus infection and uncovering the secrets of endocytosis.
PMID:
22117089
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22117089