tetano
Editor, Senior Moderator
Iran J Allergy Asthma Immunol. 2015 Aug;14(4):392-401.
[h=1]Effect of Influenza Virus Infection in a Murine Model of Asthma.[/h] Soo-Kim H[SUP]1[/SUP], Lee H[SUP]1[/SUP], Sook-Kim H[SUP]1[/SUP], Won S[SUP]1[/SUP], Kyoung-Lee E[SUP]1[/SUP], Bang K[SUP]1[/SUP], Hong-Chun Y[SUP]1[/SUP], Yoon J[SUP]1[/SUP], Hee-Kim H[SUP]1[/SUP], Tack-Kim J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Respiratory virus infection is a major cause of asthma exacerbation. However, the underlying mechanisms of this exacerbation are unknown. Therefore, to determine the mechanisms, we examined the effect of influenza infection in a murine model of asthma.Mice were divided into four groups: the phosphate-buffered saline (PBS), house dust mite(HDM), influenza, and HDM/influenza groups. The influenza group and the HDM/influenza group were infected with influenza A virus. We measured airway resistance (Penh value), examined the lung tissue for pathology, and analyzed the cells and cytokines in bronchoalveolar lavage fluid (BALF) by ELISA.At 50 mg/mL methacholine, the HDM/influenza group showed a significantly higherPenh value than the PBS, HDM, and influenza groups. The number of neutrophils in BALF was higher in the HDM/influenza group than in the HDM group. A significantly greater number of lymphocytes and macrophages were detected in the HDM/influenza group than in the HDM group. IFN-γ and IL-1β levels were higher in the HDM/influenza group than in the HDM group. IL-5 levels did not vary between the HDM and HDM/influenza groups, IL-10 was significantly lower in the HDM/influenza than in the HDM group. Chemokine (C-X-C motif) ligand 1 (CXCL1) and regulated upon activation, normal T cell expressed and secreted (RANTES) were higher in the HDM/influenza group than in the HDM group.In a murine model of asthma, influenza-induced airway inflammation appeared to becaused by simultaneous activation of neutrophilic and eosinophilic inflammation.
[h=4]KEYWORDS:[/h] Asthma; Cytokine; Eosinophil; Histology; Influenza; Mouse; Neutrophil
PMID: 26547707 [PubMed - in process]
[h=1]Effect of Influenza Virus Infection in a Murine Model of Asthma.[/h] Soo-Kim H[SUP]1[/SUP], Lee H[SUP]1[/SUP], Sook-Kim H[SUP]1[/SUP], Won S[SUP]1[/SUP], Kyoung-Lee E[SUP]1[/SUP], Bang K[SUP]1[/SUP], Hong-Chun Y[SUP]1[/SUP], Yoon J[SUP]1[/SUP], Hee-Kim H[SUP]1[/SUP], Tack-Kim J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Respiratory virus infection is a major cause of asthma exacerbation. However, the underlying mechanisms of this exacerbation are unknown. Therefore, to determine the mechanisms, we examined the effect of influenza infection in a murine model of asthma.Mice were divided into four groups: the phosphate-buffered saline (PBS), house dust mite(HDM), influenza, and HDM/influenza groups. The influenza group and the HDM/influenza group were infected with influenza A virus. We measured airway resistance (Penh value), examined the lung tissue for pathology, and analyzed the cells and cytokines in bronchoalveolar lavage fluid (BALF) by ELISA.At 50 mg/mL methacholine, the HDM/influenza group showed a significantly higherPenh value than the PBS, HDM, and influenza groups. The number of neutrophils in BALF was higher in the HDM/influenza group than in the HDM group. A significantly greater number of lymphocytes and macrophages were detected in the HDM/influenza group than in the HDM group. IFN-γ and IL-1β levels were higher in the HDM/influenza group than in the HDM group. IL-5 levels did not vary between the HDM and HDM/influenza groups, IL-10 was significantly lower in the HDM/influenza than in the HDM group. Chemokine (C-X-C motif) ligand 1 (CXCL1) and regulated upon activation, normal T cell expressed and secreted (RANTES) were higher in the HDM/influenza group than in the HDM group.In a murine model of asthma, influenza-induced airway inflammation appeared to becaused by simultaneous activation of neutrophilic and eosinophilic inflammation.
[h=4]KEYWORDS:[/h] Asthma; Cytokine; Eosinophil; Histology; Influenza; Mouse; Neutrophil
PMID: 26547707 [PubMed - in process]