tetano
Editor, Senior Moderator
Sci Rep. 2019 Aug 7;9(1):11489. doi: 10.1038/s41598-019-47866-2.
[h=1]Early life exposures shape the CD4[SUP]+[/SUP] T cell transcriptome, influencing proliferation, differentiation, and mitochondrial dynamics later in life.[/h] Burke CG[SUP]1[/SUP], Myers JR[SUP]2[/SUP], Boule LA[SUP]1[/SUP], Post CM[SUP]3[/SUP], Brookes PS[SUP]4[/SUP], Lawrence BP[SUP]5,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology & Immunology, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 2 Genomics Research Center, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 3 Department of Environmental Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 4 Department of Anesthesiology, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 5 Department of Microbiology & Immunology, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. Paige_Lawrence@URMC.Rochester.edu. 6 Department of Environmental Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. Paige_Lawrence@URMC.Rochester.edu.
[h=3]Abstract[/h] Early life environmental exposures drive lasting changes to the function of the immune system and can contribute to disease later in life. One of the ways environmental factors act is through cellular receptors. The aryl hydrocarbon receptor (AHR) is expressed by immune cells and binds numerous xenobiotics. Early life exposure to chemicals that bind the AHR impairs CD4[SUP]+[/SUP] T cell responses to influenza A virus (IAV) infection in adulthood. However, the cellular mechanisms that underlie these durable changes remain poorly defined. Transcriptomic profiling of sorted CD4[SUP]+[/SUP] T cells identified changes in genes involved in proliferation, differentiation, and metabolic pathways were associated with triggering AHR during development. Functional bioassays confirmed that CD4[SUP]+[/SUP] T cells from infected developmentally exposed offspring exhibit reduced proliferation, differentiation, and cellular metabolism. Thus, developmental AHR activation shapes T cell responsive capacity later in life by affecting integrated cellular pathways, which collectively alter responses later in life. Given that coordinated shifts in T cell metabolism are essential for T cell responses to numerous challenges, and that humans are constantly exposed to many different types of AHR ligands, this has far-reaching implications for how AHR signaling, particularly during development, durably influences T cell mediated immune responses across the lifespan.
PMID: 31391494 DOI: 10.1038/s41598-019-47866-2
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[h=1]Early life exposures shape the CD4[SUP]+[/SUP] T cell transcriptome, influencing proliferation, differentiation, and mitochondrial dynamics later in life.[/h] Burke CG[SUP]1[/SUP], Myers JR[SUP]2[/SUP], Boule LA[SUP]1[/SUP], Post CM[SUP]3[/SUP], Brookes PS[SUP]4[/SUP], Lawrence BP[SUP]5,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology & Immunology, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 2 Genomics Research Center, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 3 Department of Environmental Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 4 Department of Anesthesiology, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. 5 Department of Microbiology & Immunology, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. Paige_Lawrence@URMC.Rochester.edu. 6 Department of Environmental Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, 14624, USA. Paige_Lawrence@URMC.Rochester.edu.
[h=3]Abstract[/h] Early life environmental exposures drive lasting changes to the function of the immune system and can contribute to disease later in life. One of the ways environmental factors act is through cellular receptors. The aryl hydrocarbon receptor (AHR) is expressed by immune cells and binds numerous xenobiotics. Early life exposure to chemicals that bind the AHR impairs CD4[SUP]+[/SUP] T cell responses to influenza A virus (IAV) infection in adulthood. However, the cellular mechanisms that underlie these durable changes remain poorly defined. Transcriptomic profiling of sorted CD4[SUP]+[/SUP] T cells identified changes in genes involved in proliferation, differentiation, and metabolic pathways were associated with triggering AHR during development. Functional bioassays confirmed that CD4[SUP]+[/SUP] T cells from infected developmentally exposed offspring exhibit reduced proliferation, differentiation, and cellular metabolism. Thus, developmental AHR activation shapes T cell responsive capacity later in life by affecting integrated cellular pathways, which collectively alter responses later in life. Given that coordinated shifts in T cell metabolism are essential for T cell responses to numerous challenges, and that humans are constantly exposed to many different types of AHR ligands, this has far-reaching implications for how AHR signaling, particularly during development, durably influences T cell mediated immune responses across the lifespan.
PMID: 31391494 DOI: 10.1038/s41598-019-47866-2
Free full text