tetano
Editor, Senior Moderator
J Control Release. 2010 Aug 5. [Epub ahead of print]
Dose sparing enabled by skin immunization with influenza virus-like particle vaccine using microneedles.
Quan FS, Kim YC, Compans RW, Prausnitz MR, Kang SM.
Department of Microbiology and Immunology, and Yerkes Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA.
Abstract
To address limitations of conventional influenza vaccine manufacturing and delivery, this study investigated administration of virus-like particle (VLP) influenza vaccine using a microneedle patch. The goal was to determine if skin immunization with influenza VLP vaccine using microneedles enables dose sparing. We found that low-dose influenza (A/PR/8/34 H1N1) VLP vaccination using microneedles was more immunogenic than low-dose intramuscular (IM) vaccination and similarly immunogenic as high-dose IM vaccination in a mouse model. With 1 mug dose of vaccine, both routes showed similar immune responses and protective efficacy, with microneedle vaccination being more effective in inducing recall antibody responses in lungs and antibody secreting cells in bone marrow. With a low dose of vaccine (0.3 mug), microneedle vaccination induced significantly superior protective immunity, which included binding and functional antibodies as well as complete protection against a high dose of lethal infection with A/PR/8/34 virus, whereas IM immunization provided only partial (40%) protection. Therefore, this study demonstrates that microneedle vaccination in the skin confers more effective protective immunity at a lower dose, thus providing vaccine dose sparing effects. Copyright ? 2010. Published by Elsevier B.V.
PMID: 20692307 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20692307
Dose sparing enabled by skin immunization with influenza virus-like particle vaccine using microneedles.
Quan FS, Kim YC, Compans RW, Prausnitz MR, Kang SM.
Department of Microbiology and Immunology, and Yerkes Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA.
Abstract
To address limitations of conventional influenza vaccine manufacturing and delivery, this study investigated administration of virus-like particle (VLP) influenza vaccine using a microneedle patch. The goal was to determine if skin immunization with influenza VLP vaccine using microneedles enables dose sparing. We found that low-dose influenza (A/PR/8/34 H1N1) VLP vaccination using microneedles was more immunogenic than low-dose intramuscular (IM) vaccination and similarly immunogenic as high-dose IM vaccination in a mouse model. With 1 mug dose of vaccine, both routes showed similar immune responses and protective efficacy, with microneedle vaccination being more effective in inducing recall antibody responses in lungs and antibody secreting cells in bone marrow. With a low dose of vaccine (0.3 mug), microneedle vaccination induced significantly superior protective immunity, which included binding and functional antibodies as well as complete protection against a high dose of lethal infection with A/PR/8/34 virus, whereas IM immunization provided only partial (40%) protection. Therefore, this study demonstrates that microneedle vaccination in the skin confers more effective protective immunity at a lower dose, thus providing vaccine dose sparing effects. Copyright ? 2010. Published by Elsevier B.V.
PMID: 20692307 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20692307