tetano
Editor, Senior Moderator
Virology. 2015 Aug 25;485:252-262. doi: 10.1016/j.virol.2015.08.004. [Epub ahead of print]
[h=1]Diverse antigenic site targeting of influenza hemagglutinin in the murine antibody recall response to A(H1N1)pdm09 virus.[/h] Wilson JR[SUP]1[/SUP], Guo Z[SUP]1[/SUP], Tzeng WP[SUP]1[/SUP], Garten RJ[SUP]1[/SUP], Xiyan X[SUP]1[/SUP], Blanchard EG[SUP]1[/SUP], Blanchfield K[SUP]1[/SUP], Stevens J[SUP]1[/SUP], Katz JM[SUP]1[/SUP], York IA[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Here we define the epitopes on HA that are targeted by a group of 9 recombinant monoclonal antibodies (rmAbs) isolated from memory B cells of mice, immunized by infection with A(H1N1)pdm09 virus followed by a seasonal TIV boost. These rmAbs were all reactive against the HA1 region of HA, but display 7 distinct binding footprints, targeting each of the 4 known antigenic sites. Although the rmAbs were not broadly cross-reactive, a group showed subtype-specific cross-reactivity with the HA of A/South Carolina/1/18. Screening these rmAbs with a panel of human A(H1N1)pdm09 virus isolates indicated that naturally-occurring changes in HA could reduce rmAb binding, HI activity, and/or virus neutralization activity by rmAb, without showing changes in recognition by polyclonal antiserum. In some instances, virus neutralization was lost while both ELISA binding and HI activity were retained, demonstrating a discordance between the two serological assays traditionally used to detect antigenic drift.
Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] A/H1N1/pdm09; Antigenic drift; Antigenic site; Epitope mapping; Influenza; Monoclonal antibody; Virus neutralization
PMID: 26318247 [PubMed - as supplied by publisher]
[h=1]Diverse antigenic site targeting of influenza hemagglutinin in the murine antibody recall response to A(H1N1)pdm09 virus.[/h] Wilson JR[SUP]1[/SUP], Guo Z[SUP]1[/SUP], Tzeng WP[SUP]1[/SUP], Garten RJ[SUP]1[/SUP], Xiyan X[SUP]1[/SUP], Blanchard EG[SUP]1[/SUP], Blanchfield K[SUP]1[/SUP], Stevens J[SUP]1[/SUP], Katz JM[SUP]1[/SUP], York IA[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Here we define the epitopes on HA that are targeted by a group of 9 recombinant monoclonal antibodies (rmAbs) isolated from memory B cells of mice, immunized by infection with A(H1N1)pdm09 virus followed by a seasonal TIV boost. These rmAbs were all reactive against the HA1 region of HA, but display 7 distinct binding footprints, targeting each of the 4 known antigenic sites. Although the rmAbs were not broadly cross-reactive, a group showed subtype-specific cross-reactivity with the HA of A/South Carolina/1/18. Screening these rmAbs with a panel of human A(H1N1)pdm09 virus isolates indicated that naturally-occurring changes in HA could reduce rmAb binding, HI activity, and/or virus neutralization activity by rmAb, without showing changes in recognition by polyclonal antiserum. In some instances, virus neutralization was lost while both ELISA binding and HI activity were retained, demonstrating a discordance between the two serological assays traditionally used to detect antigenic drift.
Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] A/H1N1/pdm09; Antigenic drift; Antigenic site; Epitope mapping; Influenza; Monoclonal antibody; Virus neutralization
PMID: 26318247 [PubMed - as supplied by publisher]