Mary Wilson
Well-known member
Published: 13 June 2022
https://doi.org/10.1038/s41598-022-13396-7
Diana Cruz‐Pulido1,2, Wilberforce Zachary Ouma3 & Scott P. Kenney1,2
Abstract
Coronaviruses are important viral pathogens across a range of animal species including humans. They have a high potential for cross‐species transmission as evidenced by the emergence of COVID‐19
and may be the origin of future pandemics. There is therefore an urgent need to study coronaviruses in depth and to identify new therapeutic targets. This study shows that distant coronaviruses such as Alpha‐, Beta‐, and Deltacoronaviruses can share common host immune associated pathways and genes. Di erentially expressed genes (DEGs) in the transcription pro le of epithelial cell lines infected with swine acute diarrhea syndrome, severe acute respiratory syndrome coronavirus 2,
or porcine deltacoronavirus, showed that DEGs within 10 common immune associated pathways were upregulated upon infection. Twenty Three pathways and 21 DEGs across 10 immune response associated pathways were shared by these viruses. These 21 DEGs can serve as focused targets for therapeutics against newly emerging coronaviruses. We were able to show that even though there is a positive correlation between PDCoV and SARS‐CoV‐2 infections, these viruses could be using di erent strategies for e cient replication in their cells from their natural hosts. To the best of our knowledge, this is the rst report of comparative host transcriptome analysis across distant coronavirus genres.
https://www.nature.com/articles/s41598-022-13396-7.pdf
https://doi.org/10.1038/s41598-022-13396-7
Diana Cruz‐Pulido1,2, Wilberforce Zachary Ouma3 & Scott P. Kenney1,2
Abstract
Coronaviruses are important viral pathogens across a range of animal species including humans. They have a high potential for cross‐species transmission as evidenced by the emergence of COVID‐19
and may be the origin of future pandemics. There is therefore an urgent need to study coronaviruses in depth and to identify new therapeutic targets. This study shows that distant coronaviruses such as Alpha‐, Beta‐, and Deltacoronaviruses can share common host immune associated pathways and genes. Di erentially expressed genes (DEGs) in the transcription pro le of epithelial cell lines infected with swine acute diarrhea syndrome, severe acute respiratory syndrome coronavirus 2,
or porcine deltacoronavirus, showed that DEGs within 10 common immune associated pathways were upregulated upon infection. Twenty Three pathways and 21 DEGs across 10 immune response associated pathways were shared by these viruses. These 21 DEGs can serve as focused targets for therapeutics against newly emerging coronaviruses. We were able to show that even though there is a positive correlation between PDCoV and SARS‐CoV‐2 infections, these viruses could be using di erent strategies for e cient replication in their cells from their natural hosts. To the best of our knowledge, this is the rst report of comparative host transcriptome analysis across distant coronavirus genres.
https://www.nature.com/articles/s41598-022-13396-7.pdf