tetano
Editor, Senior Moderator
Immunol Invest. 2011 Dec 13. [Epub ahead of print]
Dietary Whole Glucan Particles Do Not Affect Antibody or Cell-Mediated Immune Responses to Influenza Virus Vaccination in Mice.
Pence BD, Hester SN, Donovan SM, Woods JA.
Source
Department of Kinesiology & Community Health, University of Illinois at Urbana-Champaign , Urbana, Illinois , USA.
Abstract
Influenza virus is a serious health concern. β-glucans derived from plants, bacteria, and fungi have been shown to potentiate immune system responses including those elicited by vaccination. However, in these studies β-glucan was administered as an adjuvant in the vaccine preparation. We hypothesized that addition of a commercially available whole glucan particle supplement to the diet would improve immune response to primary and secondary influenza vaccination in mice. β-glucan was added to pelleted diet and fed to mice at concentrations designed to deliver 0 (control), 1.8 or 90 mg?kg(-1)?day(-1) to each mouse. Influenza vaccine was given intramuscularly in the left hindlimb and primary and secondary responses were assessed. Supplementation with β-glucan was not effective in boosting immune responses to the vaccine, either in the primary or secondary vaccination experiments. Surprisingly, addition of particulate β-glucan to the vaccine itself also failed to elicit a greater antibody response. These observations suggest that this particular form of β-glucan is ineffective in boosting immune response to intramuscular influenza vaccination. Further study is warranted to determine if the use of different mouse models, different vaccine delivery systems, or β-glucans purified from different strains of bacteria, fungi, or plants could improve outcomes using this or similar protocols.
PMID:
22149961
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22149961
Dietary Whole Glucan Particles Do Not Affect Antibody or Cell-Mediated Immune Responses to Influenza Virus Vaccination in Mice.
Pence BD, Hester SN, Donovan SM, Woods JA.
Source
Department of Kinesiology & Community Health, University of Illinois at Urbana-Champaign , Urbana, Illinois , USA.
Abstract
Influenza virus is a serious health concern. β-glucans derived from plants, bacteria, and fungi have been shown to potentiate immune system responses including those elicited by vaccination. However, in these studies β-glucan was administered as an adjuvant in the vaccine preparation. We hypothesized that addition of a commercially available whole glucan particle supplement to the diet would improve immune response to primary and secondary influenza vaccination in mice. β-glucan was added to pelleted diet and fed to mice at concentrations designed to deliver 0 (control), 1.8 or 90 mg?kg(-1)?day(-1) to each mouse. Influenza vaccine was given intramuscularly in the left hindlimb and primary and secondary responses were assessed. Supplementation with β-glucan was not effective in boosting immune responses to the vaccine, either in the primary or secondary vaccination experiments. Surprisingly, addition of particulate β-glucan to the vaccine itself also failed to elicit a greater antibody response. These observations suggest that this particular form of β-glucan is ineffective in boosting immune response to intramuscular influenza vaccination. Further study is warranted to determine if the use of different mouse models, different vaccine delivery systems, or β-glucans purified from different strains of bacteria, fungi, or plants could improve outcomes using this or similar protocols.
PMID:
22149961
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22149961