tetano
Editor, Senior Moderator
J Virol Methods. 2010 May 3. [Epub ahead of print]
Development of multiplex Nucleic Acid Sequence-Based Amplification for detection of human respiratory tract viruses.
Lau LT, Feng XY, Lam TY, Hui HK, Yu AC.
Neuroscience Research Institute; Department of Neurobiology, School of Basic Medical Sciences; the Key Laboratory for Neuroscience (Ministry of Education); the Key Laboratory for Neuroscience (Ministry of Public Health); Peking University, Beijing, China; Beijing Hai Kang DNA Chips Limited, Beijing Economic Development Area, Yizhuang, Beijing, China; Infectious Disease Center, Peking University, Beijing, China; Hai Kang Life Corporation Limited, Shau Kei Wan, Hong Kong SAR, China.
Abstract
A group of common lower respiratory tract infections, influenza A, influenza B, human parainfluenza virus 1-4 (HPIV1-4), respiratory syncytial virus (RSV), rubella virus (RV) and Coxsackie virus (CSV), were selected for the development of a multiplex Nucleic Acid Sequence-Based Amplification (NASBA) assay. Quantifiable measurement utilizing an emzyme-linked oligonucleotide capture (EOC) optical detection method, which was described previously, alleviated the requirement of specialized instrumentation that is commonly used in other molecular techniques. Multiplex NASBA-EOC provided rapid and specific detection of a single virus from a multiplexed group, reducing laboratory testing time and enabling high throughput screening. The uniquely designed primers and probes proved to be highly sensitive and specific, exemplifying the robustness of the multiplex NASBA-EOC technique. Copyright ? 2010. Published by Elsevier B.V.
PMID: 20447419 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20447419?dopt=Abstract
Development of multiplex Nucleic Acid Sequence-Based Amplification for detection of human respiratory tract viruses.
Lau LT, Feng XY, Lam TY, Hui HK, Yu AC.
Neuroscience Research Institute; Department of Neurobiology, School of Basic Medical Sciences; the Key Laboratory for Neuroscience (Ministry of Education); the Key Laboratory for Neuroscience (Ministry of Public Health); Peking University, Beijing, China; Beijing Hai Kang DNA Chips Limited, Beijing Economic Development Area, Yizhuang, Beijing, China; Infectious Disease Center, Peking University, Beijing, China; Hai Kang Life Corporation Limited, Shau Kei Wan, Hong Kong SAR, China.
Abstract
A group of common lower respiratory tract infections, influenza A, influenza B, human parainfluenza virus 1-4 (HPIV1-4), respiratory syncytial virus (RSV), rubella virus (RV) and Coxsackie virus (CSV), were selected for the development of a multiplex Nucleic Acid Sequence-Based Amplification (NASBA) assay. Quantifiable measurement utilizing an emzyme-linked oligonucleotide capture (EOC) optical detection method, which was described previously, alleviated the requirement of specialized instrumentation that is commonly used in other molecular techniques. Multiplex NASBA-EOC provided rapid and specific detection of a single virus from a multiplexed group, reducing laboratory testing time and enabling high throughput screening. The uniquely designed primers and probes proved to be highly sensitive and specific, exemplifying the robustness of the multiplex NASBA-EOC technique. Copyright ? 2010. Published by Elsevier B.V.
PMID: 20447419 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20447419?dopt=Abstract