tetano
Editor, Senior Moderator
J Virol Methods. 2014 Apr 18. pii: S0166-0934(14)00151-7. doi: 10.1016/j.jviromet.2014.03.028. [Epub ahead of print]
Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection.
Nakauchi M1, Takayama I1, Takahashi H1, Tashiro M1, Kageyama T2.
Author information
Abstract
A genetic diagnosis system for detecting avian influenza A (H7N9) virus infection using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology was developed. The RT-LAMP assay showed no cross-reactivity with seasonal influenza A (H3N2 and H1N1pdm09) or influenza B viruses circulating in humans or with avian influenza A (H5N1) viruses. The sensitivity of the RT-LAMP assay was 42.47copies/reaction. Considering the high specificity and sensitivity of the assay for detecting the avian influenza A (H7N9) virus and that the reaction was completed within 30min, the RT-LAMP assay developed in this study is a promising rapid diagnostic tool for avian influenza A (H7N9) virus infection.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Avian influenza virus, H7N9, RT-LAMP, Rapid diagnosis
PMID:
24747008
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24747008
Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection.
Nakauchi M1, Takayama I1, Takahashi H1, Tashiro M1, Kageyama T2.
Author information
Abstract
A genetic diagnosis system for detecting avian influenza A (H7N9) virus infection using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology was developed. The RT-LAMP assay showed no cross-reactivity with seasonal influenza A (H3N2 and H1N1pdm09) or influenza B viruses circulating in humans or with avian influenza A (H5N1) viruses. The sensitivity of the RT-LAMP assay was 42.47copies/reaction. Considering the high specificity and sensitivity of the assay for detecting the avian influenza A (H7N9) virus and that the reaction was completed within 30min, the RT-LAMP assay developed in this study is a promising rapid diagnostic tool for avian influenza A (H7N9) virus infection.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Avian influenza virus, H7N9, RT-LAMP, Rapid diagnosis
PMID:
24747008
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24747008