tetano
Editor, Senior Moderator
J Infect Dis. 2015 Jun 11. pii: jiv331. [Epub ahead of print]
[h=1]Determination of Influenza Virus Infectivity and Correlation to Diagnostic Assays in Ferret Model.[/h] Inagaki K[SUP]1[/SUP], Song MS[SUP]1[/SUP], Crumpton JC[SUP]1[/SUP], DeBeauchamp J[SUP]1[/SUP], Jeevan T[SUP]1[/SUP], Tuomanen EI[SUP]1[/SUP], Webby RJ[SUP]1[/SUP], Hakim H[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The relationship of influenza infectivity and virus shedding by different diagnostic methods has not been defined.
[h=4]METHODS:[/h] Three donor ferrets infected with 2009 pandemic H1N1 influenza virus underwent daily quantitative culture, antigen detection, and real-time reverse-transcriptase polymerase chain reaction (rRT-PCR). Eight contacts were sequentially co-housed with each of the donors for 24 hours during 3-10 days post-inoculation (dpi).
[h=4]RESULTS:[/h] Transmission was observed until 5 dpi, corresponding to high culture titers and positive antigen detection. rRT-PCR showed no relation to the cessation of transmission.
[h=4]CONCLUSIONS:[/h] Antigen detection and virus culture, but not rRT-PCR, identify the end of infectious period.
? The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID: 26068783 [PubMed - as supplied by publisher]
[h=1]Determination of Influenza Virus Infectivity and Correlation to Diagnostic Assays in Ferret Model.[/h] Inagaki K[SUP]1[/SUP], Song MS[SUP]1[/SUP], Crumpton JC[SUP]1[/SUP], DeBeauchamp J[SUP]1[/SUP], Jeevan T[SUP]1[/SUP], Tuomanen EI[SUP]1[/SUP], Webby RJ[SUP]1[/SUP], Hakim H[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The relationship of influenza infectivity and virus shedding by different diagnostic methods has not been defined.
[h=4]METHODS:[/h] Three donor ferrets infected with 2009 pandemic H1N1 influenza virus underwent daily quantitative culture, antigen detection, and real-time reverse-transcriptase polymerase chain reaction (rRT-PCR). Eight contacts were sequentially co-housed with each of the donors for 24 hours during 3-10 days post-inoculation (dpi).
[h=4]RESULTS:[/h] Transmission was observed until 5 dpi, corresponding to high culture titers and positive antigen detection. rRT-PCR showed no relation to the cessation of transmission.
[h=4]CONCLUSIONS:[/h] Antigen detection and virus culture, but not rRT-PCR, identify the end of infectious period.
? The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID: 26068783 [PubMed - as supplied by publisher]