tetano
Editor, Senior Moderator
J Infect. 2013 Nov 6. pii: S0163-4453(13)00330-7. doi: 10.1016/j.jinf.2013.10.013. [Epub ahead of print]
Detection Of Viral And Bacterial Pathogens In Acute Respiratory Infections.
Obasi CN, Barrett B, Brown R, Vrtis R, Barlow S, Muller D, Gern J.
Source
Department of Family Medicine, University of Wisconsin-Madison, School of Medicine and Public Health. Electronic address: cobasi@wisc.edu.
Abstract
OBJECTIVES:
The role of bacteria in acute respiratory illnesses (ARI) of adults and interactions with viral infections is incompletely understood. This study tested the hypothesis that bacterial co-infection during ARI adds to airway inflammation and illness severity.
METHODS:
Two groups of 97 specimens each were randomly selected from multiplex-PCR identified virus-positive and virus-negative nasal specimens obtained from adults with new onset ARI, and 40 control specimens were collected from healthy adults. All specimens were analyzed for Haemophilus influenza(HI), Moraxella catarrhalis(MC) and Streptococcus pneumonia(SP) by quantitative-PCR. General linear models tested for relationships between respiratory pathogens, biomarkers (nasal wash neutrophils and CXCL8), and ARI-severity.
RESULTS:
Nasal specimens from adults with ARIs were more likely to contain bacteria (37% overall; HI=28%, MC=14%, SP=7%) compared to specimens from healthy adults (5% overall; HI=0%, MC=2.5%, SP=2.5%;p<0.001). Among ARI specimens, bacteria were more likely to be detected among virus-negative specimens compared to virus-positive specimens (46% vs. 27%;p=0.0046). The presence of bacteria was significantly associated with increased CXCL8 and neutrophils, but not increased symptoms.
CONCLUSION:
Pathogenic bacteria were more often detected in virus-negative ARI, and also associated with increased inflammatory biomarkers. These findings suggest the possibility that bacteria may augment virus-induced ARI and contribute to airway inflammation.
? 2013 Published by Elsevier Ltd on behalf of The British Infection Association.
KEYWORDS:
Bacteria, Common cold, Inflammation, Upper respiratory infections
PMID:
24211414
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24211414
Detection Of Viral And Bacterial Pathogens In Acute Respiratory Infections.
Obasi CN, Barrett B, Brown R, Vrtis R, Barlow S, Muller D, Gern J.
Source
Department of Family Medicine, University of Wisconsin-Madison, School of Medicine and Public Health. Electronic address: cobasi@wisc.edu.
Abstract
OBJECTIVES:
The role of bacteria in acute respiratory illnesses (ARI) of adults and interactions with viral infections is incompletely understood. This study tested the hypothesis that bacterial co-infection during ARI adds to airway inflammation and illness severity.
METHODS:
Two groups of 97 specimens each were randomly selected from multiplex-PCR identified virus-positive and virus-negative nasal specimens obtained from adults with new onset ARI, and 40 control specimens were collected from healthy adults. All specimens were analyzed for Haemophilus influenza(HI), Moraxella catarrhalis(MC) and Streptococcus pneumonia(SP) by quantitative-PCR. General linear models tested for relationships between respiratory pathogens, biomarkers (nasal wash neutrophils and CXCL8), and ARI-severity.
RESULTS:
Nasal specimens from adults with ARIs were more likely to contain bacteria (37% overall; HI=28%, MC=14%, SP=7%) compared to specimens from healthy adults (5% overall; HI=0%, MC=2.5%, SP=2.5%;p<0.001). Among ARI specimens, bacteria were more likely to be detected among virus-negative specimens compared to virus-positive specimens (46% vs. 27%;p=0.0046). The presence of bacteria was significantly associated with increased CXCL8 and neutrophils, but not increased symptoms.
CONCLUSION:
Pathogenic bacteria were more often detected in virus-negative ARI, and also associated with increased inflammatory biomarkers. These findings suggest the possibility that bacteria may augment virus-induced ARI and contribute to airway inflammation.
? 2013 Published by Elsevier Ltd on behalf of The British Infection Association.
KEYWORDS:
Bacteria, Common cold, Inflammation, Upper respiratory infections
PMID:
24211414
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24211414