tetano
Editor, Senior Moderator
J Clin Microbiol. 2011 May 18. [Epub ahead of print]
Design and Performance of the CDC Real-time RT-PCR Swine Flu Panel for Detection of 2009 A (H1N1) Pandemic Influenza Virus.
Shu B, Wu KH, Emery S, Villanueva J, Johnson R, Guthrie E, Berman L, Warnes C, Barnes N, Klimov A, Lindstrom S.
Source
Virus Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention.
Abstract
Swine influenza viruses (SIV), have been shown to sporadically infect humans, and are infrequently identified by Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypable influenza A virus samples. Real-time RT-PCR (rRT-PCR) procedures for detection and characterization of North American lineage (N.Am) SIV were developed and implemented at CDC for rapid identification of specimens from cases of suspected infections with SIV. These procedures were utilized in April 2009 for detection of human cases of 2009 A (H1N1) pandemic (pdm) influenza virus infection. Based on genetic sequence data of the first two viruses, the previously developed rRT-PCR procedures were optimized to create the CDC rRT-PCR Swine Flu Panel for detection of the 2009 A (H1N1) pdm influenza virus. Analytical sensitivity of the CDC rRT-PCR Swine Flu Panel was shown to be 5 copies of RNA per-reaction and 10(-1.3∼ -0.7) ID(50) per-reaction for cultured viruses. Cross reactivity was not observed when testing human clinical specimens or cultured viruses that were positive for human seasonal A (H1N1, H3N2) and B influenza viruses. The CDC rRT-PCR Swine Flu Panel was distributed to public health laboratories in the United States and internationally from April 2009 until June 2010. The CDC rRT-PCR Swine Flu Panel served as effective tool for timely and specific detection of 2009 A (H1N1) pdm influenza viruses and facilitated subsequent public health response implementation.
PMID:
21593260
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21593260
Design and Performance of the CDC Real-time RT-PCR Swine Flu Panel for Detection of 2009 A (H1N1) Pandemic Influenza Virus.
Shu B, Wu KH, Emery S, Villanueva J, Johnson R, Guthrie E, Berman L, Warnes C, Barnes N, Klimov A, Lindstrom S.
Source
Virus Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention.
Abstract
Swine influenza viruses (SIV), have been shown to sporadically infect humans, and are infrequently identified by Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypable influenza A virus samples. Real-time RT-PCR (rRT-PCR) procedures for detection and characterization of North American lineage (N.Am) SIV were developed and implemented at CDC for rapid identification of specimens from cases of suspected infections with SIV. These procedures were utilized in April 2009 for detection of human cases of 2009 A (H1N1) pandemic (pdm) influenza virus infection. Based on genetic sequence data of the first two viruses, the previously developed rRT-PCR procedures were optimized to create the CDC rRT-PCR Swine Flu Panel for detection of the 2009 A (H1N1) pdm influenza virus. Analytical sensitivity of the CDC rRT-PCR Swine Flu Panel was shown to be 5 copies of RNA per-reaction and 10(-1.3∼ -0.7) ID(50) per-reaction for cultured viruses. Cross reactivity was not observed when testing human clinical specimens or cultured viruses that were positive for human seasonal A (H1N1, H3N2) and B influenza viruses. The CDC rRT-PCR Swine Flu Panel was distributed to public health laboratories in the United States and internationally from April 2009 until June 2010. The CDC rRT-PCR Swine Flu Panel served as effective tool for timely and specific detection of 2009 A (H1N1) pdm influenza viruses and facilitated subsequent public health response implementation.
PMID:
21593260
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21593260