tetano
Editor, Senior Moderator
Pharmaceuticals (Basel). 2017 Aug 9;10(3). pii: E71. doi: 10.3390/ph10030071.
[h=1]Complexes of Oligoribonucleotides with D-Mannitol Inhibit Hemagglutinin-Glycan Interaction and Suppress Influenza A Virus H1N1 (A/FM/1/47) Infectivity In Vitro.[/h] Melnichuk N[SUP]1[/SUP], Semernikova L[SUP]2[/SUP], Tkachuk Z[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza virus hemagglutinin (HA) mediates both receptor (glycan) binding and membrane fusion for cell entry and has been the basis for subtyping influenza viruses. The oligoribonucleotides-d-mannitol (ORNs-d-M) complexes possess an anti-influenza activity in vitro and in vivo. In the present studies, we have found that ORNs-d-M interferes with hemagglutinin (HA)-glycan interaction and suppress viral infection in host cells. HA-glycan interactions were evaluated to indirectly quantify the amount of influenza virus titer by an agglutination assay. Influenza virus infectivity was determined by TCID[SUB]50[/SUB] assay. The direct virucidal action of the complexes was evaluated by both cytopathic effects (CPE) reduction assay and cell MTT assay. We found that ORNs-d-M hinders interaction between HA and glycan. These complexes decreased the infectivity of influenza virus and had a direct virucidal action. ORNs-d-M reduces influenza virus infectivity, affecting the HA-glycan interaction in vitro. By suppressing the influenza viral infection, the ORNs-d-M can have direct virucidal action.
[h=4]KEYWORDS:[/h] ">d-mannitol complexes; hemagglutinin; influenza virus; oligoribonucleotides-
PMID: 28792452 DOI: 10.3390/ph10030071
Free full text
[h=1]Complexes of Oligoribonucleotides with D-Mannitol Inhibit Hemagglutinin-Glycan Interaction and Suppress Influenza A Virus H1N1 (A/FM/1/47) Infectivity In Vitro.[/h] Melnichuk N[SUP]1[/SUP], Semernikova L[SUP]2[/SUP], Tkachuk Z[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza virus hemagglutinin (HA) mediates both receptor (glycan) binding and membrane fusion for cell entry and has been the basis for subtyping influenza viruses. The oligoribonucleotides-d-mannitol (ORNs-d-M) complexes possess an anti-influenza activity in vitro and in vivo. In the present studies, we have found that ORNs-d-M interferes with hemagglutinin (HA)-glycan interaction and suppress viral infection in host cells. HA-glycan interactions were evaluated to indirectly quantify the amount of influenza virus titer by an agglutination assay. Influenza virus infectivity was determined by TCID[SUB]50[/SUB] assay. The direct virucidal action of the complexes was evaluated by both cytopathic effects (CPE) reduction assay and cell MTT assay. We found that ORNs-d-M hinders interaction between HA and glycan. These complexes decreased the infectivity of influenza virus and had a direct virucidal action. ORNs-d-M reduces influenza virus infectivity, affecting the HA-glycan interaction in vitro. By suppressing the influenza viral infection, the ORNs-d-M can have direct virucidal action.
[h=4]KEYWORDS:[/h] ">d-mannitol complexes; hemagglutinin; influenza virus; oligoribonucleotides-
PMID: 28792452 DOI: 10.3390/ph10030071
Free full text