tetano
Editor, Senior Moderator
J Virol. 2013 Jun 26. [Epub ahead of print]
Complex reassortment of multiple subtypes of avian influenza viruses in domestic ducks at the Dongting Lake region of China.
Deng G, Tan D, Shi J, Cui P, Jiang Y, Liu L, Tian G, Kawaoka Y, Li C, Chen H.
Source
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Abstract
To gain insight into the ecology of avian influenza viruses (AIV), we conducted active influenza virus surveillance in domestic ducks in farms located on the flyway of migratory birds in the Dongting Lake region of Hunan province, China from the winter of 2011 until spring of 2012. Specimens of 3030 duck swab samples and 1010 environmental samples were collected from 101 duck farms. We isolated AIV of various HA subtypes, including H3, H4, H5, H6, H9, H10, H11, and H12. We sequenced the entire coding sequences of the genomes of 28 representative isolates that constitute 13 specific subtypes. When the phylogenetic relationships among these isolates were examined, we observed that extensive reassortment events had occurred. Of the 28 Dongting Lake viruses, 21 genotypes involving the six internal genes were identified. Furthermore, we identified viruses or viral genes introduced from other countries, viral gene segments of unknown origin, and a novel HA/NA combination. Our findings emphasize the importance of farmed domestic ducks at Dongting Lake to the genesis and evolution of AIV and highlight the need for continued surveillance of domestic ducks in this region.
PMID:
23804642
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23804642
Complex reassortment of multiple subtypes of avian influenza viruses in domestic ducks at the Dongting Lake region of China.
Deng G, Tan D, Shi J, Cui P, Jiang Y, Liu L, Tian G, Kawaoka Y, Li C, Chen H.
Source
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Abstract
To gain insight into the ecology of avian influenza viruses (AIV), we conducted active influenza virus surveillance in domestic ducks in farms located on the flyway of migratory birds in the Dongting Lake region of Hunan province, China from the winter of 2011 until spring of 2012. Specimens of 3030 duck swab samples and 1010 environmental samples were collected from 101 duck farms. We isolated AIV of various HA subtypes, including H3, H4, H5, H6, H9, H10, H11, and H12. We sequenced the entire coding sequences of the genomes of 28 representative isolates that constitute 13 specific subtypes. When the phylogenetic relationships among these isolates were examined, we observed that extensive reassortment events had occurred. Of the 28 Dongting Lake viruses, 21 genotypes involving the six internal genes were identified. Furthermore, we identified viruses or viral genes introduced from other countries, viral gene segments of unknown origin, and a novel HA/NA combination. Our findings emphasize the importance of farmed domestic ducks at Dongting Lake to the genesis and evolution of AIV and highlight the need for continued surveillance of domestic ducks in this region.
PMID:
23804642
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23804642