tetano
Editor, Senior Moderator
J Virol. 2012 Jun 27. [Epub ahead of print]
Competitive incorporation of homologous gene segments of influenza A virus into virions.
Inagaki A, Goto H, Kakugawa S, Ozawa M, Kawaoka Y.
Source
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.
Abstract
By using two reporter gene-encoding virus-like RNAs derived from identical vRNA segments, we assessed their incorporation efficiency into single progeny virions. Most plaques formed by the recombinant viruses that were generated in cells positive for both reporter genes expressed only one or the other protein. These results suggest that two virus-like RNAs encoding different reporter proteins compete for incorporation into virions, and individual influenza virions incorporate single, but not multiple, copies of homologous vRNA segments.
PMID:
22740412
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22740412
Competitive incorporation of homologous gene segments of influenza A virus into virions.
Inagaki A, Goto H, Kakugawa S, Ozawa M, Kawaoka Y.
Source
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.
Abstract
By using two reporter gene-encoding virus-like RNAs derived from identical vRNA segments, we assessed their incorporation efficiency into single progeny virions. Most plaques formed by the recombinant viruses that were generated in cells positive for both reporter genes expressed only one or the other protein. These results suggest that two virus-like RNAs encoding different reporter proteins compete for incorporation into virions, and individual influenza virions incorporate single, but not multiple, copies of homologous vRNA segments.
PMID:
22740412
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22740412