tetano
Editor, Senior Moderator
Vet Microbiol. 2014 Dec 31. pii: S0378-1135(14)00594-X. doi: 10.1016/j.vetmic.2014.12.021. [Epub ahead of print]
[h=1]Comparative virulence of wild-type H1N1pdm09 influenza A isolates in swine.[/h] Henningson JN[SUP]1[/SUP], Rajao DS[SUP]2[/SUP], Kitikoon P[SUP]2[/SUP], Lorusso A[SUP]3[/SUP], Culhane MR[SUP]4[/SUP], Lewis NS[SUP]5[/SUP], Anderson TK[SUP]6[/SUP], Vincent AL[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In 2009, a novel swine-origin H1N1 (H1N1pdm09) influenza A virus (IAV) reached pandemic status and was soon after detected in pigs worldwide. The objective of this study was to evaluate whether differences in the HA protein can affect pathogenicity and antigenicity of H1N1pdm09 in swine. We compared lung pathology, viral replication and shedding and the antigenic relationships of four wild-type H1N1pdm09 viruses in pigs: one human (CA/09) and three isolated in swine after the pandemic (IL/09, IL/10, and MN/10). The swine strains were selected based upon unique amino acid substitutions in the HA protein. All selected viruses resulted in mild disease and viral shedding through nasal and oral fluids, however, viral replication and the degree of pathology varied between the isolates. A/Swine/IL/5265/2010 (IL/10), with substitutions I120M, S146G, S186P, V252M, had lower viral titers in the lungs and nasal secretions and fewer lung lesions. The other two swine viruses caused respiratory pathology and replicated to titers similar to the human CA/09, although MN/10 (with mutations D45Y, K304E, A425S) had lower nasal shedding. Swine-adapted H1N1pdm09 have zoonotic potential, and have reassorted with other co-circulating swine viruses, influencing the evolution of IAV in swine globally. Further, our results suggest that amino acid changes in the HA gene have the potential to alter the virulence of H1N1pdm09 in swine. Importantly, the limited clinical signs in pigs could result in continued circulation of these viruses with other endemic swine IAVs providing opportunities for reassortment.
Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Cross-reactivity; HI; Influenza A virus; Pandemic; Pathology; Swine
PMID: 25601799 [PubMed - as supplied by publisher]
[h=1]Comparative virulence of wild-type H1N1pdm09 influenza A isolates in swine.[/h] Henningson JN[SUP]1[/SUP], Rajao DS[SUP]2[/SUP], Kitikoon P[SUP]2[/SUP], Lorusso A[SUP]3[/SUP], Culhane MR[SUP]4[/SUP], Lewis NS[SUP]5[/SUP], Anderson TK[SUP]6[/SUP], Vincent AL[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In 2009, a novel swine-origin H1N1 (H1N1pdm09) influenza A virus (IAV) reached pandemic status and was soon after detected in pigs worldwide. The objective of this study was to evaluate whether differences in the HA protein can affect pathogenicity and antigenicity of H1N1pdm09 in swine. We compared lung pathology, viral replication and shedding and the antigenic relationships of four wild-type H1N1pdm09 viruses in pigs: one human (CA/09) and three isolated in swine after the pandemic (IL/09, IL/10, and MN/10). The swine strains were selected based upon unique amino acid substitutions in the HA protein. All selected viruses resulted in mild disease and viral shedding through nasal and oral fluids, however, viral replication and the degree of pathology varied between the isolates. A/Swine/IL/5265/2010 (IL/10), with substitutions I120M, S146G, S186P, V252M, had lower viral titers in the lungs and nasal secretions and fewer lung lesions. The other two swine viruses caused respiratory pathology and replicated to titers similar to the human CA/09, although MN/10 (with mutations D45Y, K304E, A425S) had lower nasal shedding. Swine-adapted H1N1pdm09 have zoonotic potential, and have reassorted with other co-circulating swine viruses, influencing the evolution of IAV in swine globally. Further, our results suggest that amino acid changes in the HA gene have the potential to alter the virulence of H1N1pdm09 in swine. Importantly, the limited clinical signs in pigs could result in continued circulation of these viruses with other endemic swine IAVs providing opportunities for reassortment.
Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Cross-reactivity; HI; Influenza A virus; Pandemic; Pathology; Swine
PMID: 25601799 [PubMed - as supplied by publisher]