tetano
Editor, Senior Moderator
Arch Virol. 2019 Nov 19. doi: 10.1007/s00705-019-04465-w. [Epub ahead of print] [h=1]Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase.[/h]
Evseenko VA[SUP]1[/SUP], Svyatchenko SV[SUP]2[/SUP], Kolosova NP[SUP]2[/SUP], Kovrizhkina VL[SUP]2[/SUP], Marchenko VY[SUP]2[/SUP], Durymanov AG[SUP]2[/SUP], Goncharova NI[SUP]2[/SUP], Ryzhikov AB[SUP]2[/SUP].
[h=3]Author information[/h] 1 State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk Region, 630559, Russian Federation. evseenko_va@vector.nsc.ru. 2 State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk Region, 630559, Russian Federation.
[h=3]Abstract[/h] Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT[SUB]50[/SUB]) was observed with H3N8 avian influenza virus (74 ?C), and the lowest IT[SUB]50[/SUB] was observed with the seasonal human H3N2 virus (45.5 ?C). The IT[SUB]50[/SUB] values of A(H1N1)pdm09 viruses 56.4-58.5 ?C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 ?C). An analysis of Ca[SUP]2+[/SUP] binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.
PMID: 31745716 DOI: 10.1007/s00705-019-04465-w
Evseenko VA[SUP]1[/SUP], Svyatchenko SV[SUP]2[/SUP], Kolosova NP[SUP]2[/SUP], Kovrizhkina VL[SUP]2[/SUP], Marchenko VY[SUP]2[/SUP], Durymanov AG[SUP]2[/SUP], Goncharova NI[SUP]2[/SUP], Ryzhikov AB[SUP]2[/SUP].
[h=3]Author information[/h] 1 State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk Region, 630559, Russian Federation. evseenko_va@vector.nsc.ru. 2 State Research Center of Virology and Biotechnology "Vector", Rospotrebnadzor, Koltsovo, Novosibirsk Region, 630559, Russian Federation.
[h=3]Abstract[/h] Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT[SUB]50[/SUB]) was observed with H3N8 avian influenza virus (74 ?C), and the lowest IT[SUB]50[/SUB] was observed with the seasonal human H3N2 virus (45.5 ?C). The IT[SUB]50[/SUB] values of A(H1N1)pdm09 viruses 56.4-58.5 ?C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 ?C). An analysis of Ca[SUP]2+[/SUP] binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.
PMID: 31745716 DOI: 10.1007/s00705-019-04465-w