tetano
Editor, Senior Moderator
Clin Infect Dis
. 2020 Jul 9;ciaa941.
doi: 10.1093/cid/ciaa941. Online ahead of print.
A Confirmed Case of SARS-CoV-2 Pneumonia With Routine RT-PCR Negative and Virus Variation in Guangzhou, China
Zhengtu Li[SUP] 1 [/SUP], Yinhu Li[SUP] 2 [/SUP], Lingdan Chen[SUP] 1 [/SUP], Shaoqiang Li[SUP] 1 [/SUP], Le Yu[SUP] 2 [/SUP], Airu Zhu[SUP] 1 [/SUP], Feng Yang[SUP] 1 [/SUP], Qian Jiang[SUP] 1 [/SUP], Liyan Chen[SUP] 1 [/SUP], Jincun Zhao[SUP] 1 [/SUP], Wenju Lu[SUP] 1 [/SUP], Nanshan Zhong[SUP] 1 [/SUP], Feng Ye[SUP] 1 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine RT-PCR detection of SARS-CoV-2. However, we report a confirmed case of SARS-CoV-2 pneumonia with routine RT-PCR negative. This case has been finally diagnosed by Nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variation of SARS-CoV-2 were found. This case has highlighted that false negative results could be present in the routine RT-PCR diagnosis, especially with virus variation. At the moment, Nanopore pathogen sequencing and antibody detection have been found effective in clinical diagnosis.
Keywords: Nanopore sequencing; Pneumonia; Routine RT-PCR; SARS-CoV-2; Virus variation.
. 2020 Jul 9;ciaa941.
doi: 10.1093/cid/ciaa941. Online ahead of print.
A Confirmed Case of SARS-CoV-2 Pneumonia With Routine RT-PCR Negative and Virus Variation in Guangzhou, China
Zhengtu Li[SUP] 1 [/SUP], Yinhu Li[SUP] 2 [/SUP], Lingdan Chen[SUP] 1 [/SUP], Shaoqiang Li[SUP] 1 [/SUP], Le Yu[SUP] 2 [/SUP], Airu Zhu[SUP] 1 [/SUP], Feng Yang[SUP] 1 [/SUP], Qian Jiang[SUP] 1 [/SUP], Liyan Chen[SUP] 1 [/SUP], Jincun Zhao[SUP] 1 [/SUP], Wenju Lu[SUP] 1 [/SUP], Nanshan Zhong[SUP] 1 [/SUP], Feng Ye[SUP] 1 [/SUP]
Affiliations
- PMID: 32642757
- DOI: 10.1093/cid/ciaa941
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine RT-PCR detection of SARS-CoV-2. However, we report a confirmed case of SARS-CoV-2 pneumonia with routine RT-PCR negative. This case has been finally diagnosed by Nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variation of SARS-CoV-2 were found. This case has highlighted that false negative results could be present in the routine RT-PCR diagnosis, especially with virus variation. At the moment, Nanopore pathogen sequencing and antibody detection have been found effective in clinical diagnosis.
Keywords: Nanopore sequencing; Pneumonia; Routine RT-PCR; SARS-CoV-2; Virus variation.