tetano
Editor, Senior Moderator
Clin Exp Vaccine Res
. 2022 Sep;11(3):285-289.
doi: 10.7774/cevr.2022.11.3.285. Epub 2022 Sep 30.
Recombinant proteins of spike protein of SARS-CoV-2 with the Omicron receptor-binding domain induce production of highly Omicron-specific neutralizing antibodies
Hyangju Kang[SUP] 1 [/SUP], Daniel Kim[SUP] 1 [/SUP], Kyungmin Min[SUP] 1 [/SUP], Minhee Park[SUP] 1 [/SUP], Seok-Hyun Kim[SUP] 2 [/SUP], Eun-Ju Sohn[SUP] 1 [/SUP], Bo-Hwa Choi[SUP] 1 [/SUP], Inhwan Hwang[SUP] 3 [/SUP]
Affiliations
Abstract
Various vaccines have been developed to fight severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the coronavirus disease 2019 pandemic. However, new variants of SARS-CoV-2 undermine the effort to fight SARS-CoV-2. Here, we produced S proteins harboring the receptor-binding domain (RBD) of the Omicron variant in plants. Plant-produced S proteins together with adjuvant CIA09A triggered strong immune responses in mice. Antibodies in serum inhibited interaction of recombinant human angiotensin-converting enzyme 2 with RBD of the Omicron variant, but not RBD of other variants. These results suggest that antibodies induced by RBD of the Omicron variant are highly specific for the Omicron RBD, but not for that of other variants.
Keywords: Nicotiana benthamiana; Omicron variant; Recombinant S protein; SARS-CoV-2; Vaccine.
. 2022 Sep;11(3):285-289.
doi: 10.7774/cevr.2022.11.3.285. Epub 2022 Sep 30.
Recombinant proteins of spike protein of SARS-CoV-2 with the Omicron receptor-binding domain induce production of highly Omicron-specific neutralizing antibodies
Hyangju Kang[SUP] 1 [/SUP], Daniel Kim[SUP] 1 [/SUP], Kyungmin Min[SUP] 1 [/SUP], Minhee Park[SUP] 1 [/SUP], Seok-Hyun Kim[SUP] 2 [/SUP], Eun-Ju Sohn[SUP] 1 [/SUP], Bo-Hwa Choi[SUP] 1 [/SUP], Inhwan Hwang[SUP] 3 [/SUP]
Affiliations
- PMID: 36451664
- PMCID: PMC9691866
- DOI: 10.7774/cevr.2022.11.3.285
Abstract
Various vaccines have been developed to fight severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the coronavirus disease 2019 pandemic. However, new variants of SARS-CoV-2 undermine the effort to fight SARS-CoV-2. Here, we produced S proteins harboring the receptor-binding domain (RBD) of the Omicron variant in plants. Plant-produced S proteins together with adjuvant CIA09A triggered strong immune responses in mice. Antibodies in serum inhibited interaction of recombinant human angiotensin-converting enzyme 2 with RBD of the Omicron variant, but not RBD of other variants. These results suggest that antibodies induced by RBD of the Omicron variant are highly specific for the Omicron RBD, but not for that of other variants.
Keywords: Nicotiana benthamiana; Omicron variant; Recombinant S protein; SARS-CoV-2; Vaccine.