tetano
Editor, Senior Moderator
Exp Mol Pathol. 2015 Jul 3. pii: S0014-4800(15)00141-0. doi: 10.1016/j.yexmp.2015.07.001. [Epub ahead of print]
[h=1]Circulating levels of miR-150 are associated with poorer outcomes of A/H1N1 infection.[/h] Mor?n J[SUP]1[/SUP], Ram?rez G[SUP]2[/SUP], Jim?nez L[SUP]1[/SUP], Cruz A[SUP]1[/SUP], P?rez-Patrigeon S[SUP]3[/SUP], Hidalgo A[SUP]4[/SUP], Orozco L[SUP]4[/SUP], Mart?nez A[SUP]4[/SUP], Padilla L[SUP]5[/SUP], Avila-Moreno F[SUP]6[/SUP], Cabello C[SUP]1[/SUP], Granados J[SUP]7[/SUP], Ortiz-Quintero B[SUP]1[/SUP], Ram?rez-Venegas A[SUP]1[/SUP], Ruiz-Palacios GM[SUP]3[/SUP], Zlotnik A[SUP]8[/SUP], Merino E[SUP]9[/SUP], Z??iga J[SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Overproduction of pro-inflammatory cytokines and chemokines is frequently associated with severe clinical manifestations in A/H1N1. Micro-RNAs (miRNAs) are highly conserved small non-coding RNA molecules that post-transcriptionally regulate gene expression and are potential biomarkers and therapeutic targets in different inflammatory conditions METHODS: We studied the circulating and miRNA profiles in critically ill A/H1N1 patients, A/H1N1 patients with milder disease, asymptomatic housemates and healthy controls. Cytokine, chemokine and growth factors that were potential targets of differentially expressed miRNAs were assessed. Kyoto Encyclopedia of Genes and Genomes (KEEG) pathways enrichment and interactome analysis of these miRNAs were also performed RESULTS: Critically ill patients exhibited a significant over-expression of circulating miR-150 (p<0.005) when compared to patients with milder disease. miR-29c and miR-145 and miR-22 are differentially expressed in patients with severe A/H1N1 disease whereas miR-210, miR-126 and miR-222 are downregulated in individuals exposed to the A/H1N1 virus. Significant correlations (p<0.05) between circulating levels of miR-150 with IL-1ra, IL-2, IL-6, CXCL8, IFN-γ, CXCL10 and G-CSF were detected, particularly in critically ill patients CONCLUSION: The up-regulation of miR-150 is associated with poorer outcomes of A/H1N1 infection. The differential expression of miRNAs related with immune processes in severe A/H1N1 disease support the potential role of these miRNAs as biomarkers of disease progression.
Copyright ? 2015. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Influenza; circulating microRNAs; critically ill patients; miR-150; miRNAs; pandemic influenza A/H1N1
PMID: 26148929 [PubMed - as supplied by publisher]
[h=1]Circulating levels of miR-150 are associated with poorer outcomes of A/H1N1 infection.[/h] Mor?n J[SUP]1[/SUP], Ram?rez G[SUP]2[/SUP], Jim?nez L[SUP]1[/SUP], Cruz A[SUP]1[/SUP], P?rez-Patrigeon S[SUP]3[/SUP], Hidalgo A[SUP]4[/SUP], Orozco L[SUP]4[/SUP], Mart?nez A[SUP]4[/SUP], Padilla L[SUP]5[/SUP], Avila-Moreno F[SUP]6[/SUP], Cabello C[SUP]1[/SUP], Granados J[SUP]7[/SUP], Ortiz-Quintero B[SUP]1[/SUP], Ram?rez-Venegas A[SUP]1[/SUP], Ruiz-Palacios GM[SUP]3[/SUP], Zlotnik A[SUP]8[/SUP], Merino E[SUP]9[/SUP], Z??iga J[SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Overproduction of pro-inflammatory cytokines and chemokines is frequently associated with severe clinical manifestations in A/H1N1. Micro-RNAs (miRNAs) are highly conserved small non-coding RNA molecules that post-transcriptionally regulate gene expression and are potential biomarkers and therapeutic targets in different inflammatory conditions METHODS: We studied the circulating and miRNA profiles in critically ill A/H1N1 patients, A/H1N1 patients with milder disease, asymptomatic housemates and healthy controls. Cytokine, chemokine and growth factors that were potential targets of differentially expressed miRNAs were assessed. Kyoto Encyclopedia of Genes and Genomes (KEEG) pathways enrichment and interactome analysis of these miRNAs were also performed RESULTS: Critically ill patients exhibited a significant over-expression of circulating miR-150 (p<0.005) when compared to patients with milder disease. miR-29c and miR-145 and miR-22 are differentially expressed in patients with severe A/H1N1 disease whereas miR-210, miR-126 and miR-222 are downregulated in individuals exposed to the A/H1N1 virus. Significant correlations (p<0.05) between circulating levels of miR-150 with IL-1ra, IL-2, IL-6, CXCL8, IFN-γ, CXCL10 and G-CSF were detected, particularly in critically ill patients CONCLUSION: The up-regulation of miR-150 is associated with poorer outcomes of A/H1N1 infection. The differential expression of miRNAs related with immune processes in severe A/H1N1 disease support the potential role of these miRNAs as biomarkers of disease progression.
Copyright ? 2015. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Influenza; circulating microRNAs; critically ill patients; miR-150; miRNAs; pandemic influenza A/H1N1
PMID: 26148929 [PubMed - as supplied by publisher]