Giuseppe
Emeritus
[Source: Chest, full page: (LINK). Abstract, edited.]
Original Research | September 2013
Repair of Lipopolysaccharide-Induced Acute Lung Injury in Mice by Endothelial Progenitor Cells, Alone and in Combination With Simvastatin
Hao Li, MD; Yong Qiang, MMed; Lian Wang, MD; Gaoming Wang, MMed; Jun Yi, MD; Hua Jing, MMed; Haiwei Wu, MD
Author and Funding Information: From the Department of Cardiothoracic Surgery (Drs Li, Qiang, L. Wang, Yi, and Wu and Prof Jing), Jinling Hospital, Clinical Medicine School of Nanjing University, Nanjing; and the Department of Cardiothoracic Surgery (Dr G. Wang), Xuzhou Central Hospital, Xuzhou, China.
Correspondence to: Haiwei Wu, MD, 305 Zhongshan E Rd, Nanjing, China; e-mail: wuhaiweicts@163.com
Funding/Support: This study was supported by research grant from the Natural Science Foundation of China [30972969].
Reproduction of this article is prohibited without written permission from the American College of Chest Physicians. See online for more details.
Chest. 2013;144(3):876-886. doi:10.1378/chest.12-2429. Published online.
Abstract
Background:
Endothelial progenitor cells (EPCs) are involved in endothelium repair of acute lung injury (ALI). Numerous studies have demonstrated that statins can promote EPC function in vitro and in vivo; therefore, the purpose of this study was to determine whether simvastatin enhances the function of EPCs participating in the repair of ALI.
Methods:
BALB/C mice were initially pretreated with simvastatin by intraperitoneal administration 24 h before, and again at the time of, intratracheal instillation of lipopolysaccharide (LPS) and subsequently treated with EPCs by IV transplantation 2 h later. The effects of capillary permeability, endothelium repair, and inflammatory cytokines were measured.
Results:
This study revealed that both simvastatin administration and EPC transplantation can reduce the severity of LPS-induced ALI in mice, and the effect can be further improved by combining the two therapies.
Conclusions:
The administration of simvastatin and EPC transplantation can reduce the severity of LPS-induced ALI in mice, and improvement is moderately enhanced in some respects when EPC transplantation is combined with simvastatin administration. The beneficial role of simvastatin on EPCs may be a component of its pleiotropic effects. Although the exact mechanism remains unknown, the combined administration of simvastatin and EPC transplantation may be a potentially important, cell-based, inflammation-mediated therapy for patients with ALI/ARDS.
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Original Research | September 2013
Repair of Lipopolysaccharide-Induced Acute Lung Injury in Mice by Endothelial Progenitor Cells, Alone and in Combination With Simvastatin
Hao Li, MD; Yong Qiang, MMed; Lian Wang, MD; Gaoming Wang, MMed; Jun Yi, MD; Hua Jing, MMed; Haiwei Wu, MD
Author and Funding Information: From the Department of Cardiothoracic Surgery (Drs Li, Qiang, L. Wang, Yi, and Wu and Prof Jing), Jinling Hospital, Clinical Medicine School of Nanjing University, Nanjing; and the Department of Cardiothoracic Surgery (Dr G. Wang), Xuzhou Central Hospital, Xuzhou, China.
Correspondence to: Haiwei Wu, MD, 305 Zhongshan E Rd, Nanjing, China; e-mail: wuhaiweicts@163.com
Funding/Support: This study was supported by research grant from the Natural Science Foundation of China [30972969].
Reproduction of this article is prohibited without written permission from the American College of Chest Physicians. See online for more details.
Chest. 2013;144(3):876-886. doi:10.1378/chest.12-2429. Published online.
Abstract
Background:
Endothelial progenitor cells (EPCs) are involved in endothelium repair of acute lung injury (ALI). Numerous studies have demonstrated that statins can promote EPC function in vitro and in vivo; therefore, the purpose of this study was to determine whether simvastatin enhances the function of EPCs participating in the repair of ALI.
Methods:
BALB/C mice were initially pretreated with simvastatin by intraperitoneal administration 24 h before, and again at the time of, intratracheal instillation of lipopolysaccharide (LPS) and subsequently treated with EPCs by IV transplantation 2 h later. The effects of capillary permeability, endothelium repair, and inflammatory cytokines were measured.
Results:
This study revealed that both simvastatin administration and EPC transplantation can reduce the severity of LPS-induced ALI in mice, and the effect can be further improved by combining the two therapies.
Conclusions:
The administration of simvastatin and EPC transplantation can reduce the severity of LPS-induced ALI in mice, and improvement is moderately enhanced in some respects when EPC transplantation is combined with simvastatin administration. The beneficial role of simvastatin on EPCs may be a component of its pleiotropic effects. Although the exact mechanism remains unknown, the combined administration of simvastatin and EPC transplantation may be a potentially important, cell-based, inflammation-mediated therapy for patients with ALI/ARDS.
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