tetano
Editor, Senior Moderator
Transbound Emerg Dis. 2019 Oct 25. doi: 10.1111/tbed.13398. [Epub ahead of print] [h=1]Characterization of H7N9 avian influenza viruses isolated from duck meat products.[/h]
Wu L[SUP]1[/SUP], Mitake H[SUP]1[/SUP], Kiso M[SUP]1[/SUP], Ito M[SUP]1[/SUP], Iwatsuki-Hirimoto K[SUP]1[/SUP], Yamayoshi S[SUP]1[/SUP], Lopes TJS[SUP]1,[/SUP][SUP]2[/SUP], Feng H[SUP]1[/SUP], Sumiyoshi R[SUP]3[/SUP], Shibata A[SUP]3[/SUP], Osaka H[SUP]3[/SUP], Imai M[SUP]1[/SUP], Watanabe T[SUP]1[/SUP], Kawaoka Y[SUP]1,[/SUP][SUP]2,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan. 2 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA. 3 Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Aichi, Japan. 4 Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
[h=3]Abstract[/h] Avian influenza H7N9 viruses have caused five epidemic waves of human infections since the first human cases were reported in 2013. In 2016, the initial low pathogenic avian influenza (LPAI) H7N9 viruses became highly pathogenic, acquiring multi-basic amino acids at the hemagglutinin cleavage site. This highly pathogenic avian influenza (HPAI) H7N9 viruses have been detected in poultry and humans in China, causing concerns of a serious threat to global public health. In Japan, both HPAI and LPAI H7N9 viruses were isolated from duck meat products carried illegally and relinquished voluntarily at the border by passengers on flights from China to Japan between 2016 and 2017. Some of the LPAI and HPAI H7N9 viruses detected at the border in Japan were characterized previously in chickens and ducks; however, their pathogenicity and replicative ability in mammals remain unknown. In this study, we assessed the biological features of two HPAI H7N9 virus isolates [A/duck/Japan/AQ-HE29-22/2017 (HE29-22) and A/duck/Japan/AQ-HE29-52/2017 (HE29-52); both of these viruses were isolated from duck meat at the border)] and an LPAI H7N9 virus isolate [A/duck/Japan/AQ-HE28-3/2016 (HE28-3)] in mice and ferrets. In mice, HE29-52 was more pathogenic than HE29-22 and HE28-3. In ferrets, the two HPAI virus isolates replicated more efficiently in the lower respiratory tract of the animals than did the LPAI virus isolate. Our results indicate that HPAI H7N9 viruses with potential to cause severe diseases in mammals have been illegally introduced to Japan.
? 2019 Blackwell Verlag GmbH.
[h=4]KEYWORDS:[/h] H7N9; Highly pathogenic avian influenza; pathogenicity in mammals
PMID: 31650680 DOI: 10.1111/tbed.13398
Wu L[SUP]1[/SUP], Mitake H[SUP]1[/SUP], Kiso M[SUP]1[/SUP], Ito M[SUP]1[/SUP], Iwatsuki-Hirimoto K[SUP]1[/SUP], Yamayoshi S[SUP]1[/SUP], Lopes TJS[SUP]1,[/SUP][SUP]2[/SUP], Feng H[SUP]1[/SUP], Sumiyoshi R[SUP]3[/SUP], Shibata A[SUP]3[/SUP], Osaka H[SUP]3[/SUP], Imai M[SUP]1[/SUP], Watanabe T[SUP]1[/SUP], Kawaoka Y[SUP]1,[/SUP][SUP]2,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan. 2 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA. 3 Exotic Disease Inspection Division, Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Aichi, Japan. 4 Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
[h=3]Abstract[/h] Avian influenza H7N9 viruses have caused five epidemic waves of human infections since the first human cases were reported in 2013. In 2016, the initial low pathogenic avian influenza (LPAI) H7N9 viruses became highly pathogenic, acquiring multi-basic amino acids at the hemagglutinin cleavage site. This highly pathogenic avian influenza (HPAI) H7N9 viruses have been detected in poultry and humans in China, causing concerns of a serious threat to global public health. In Japan, both HPAI and LPAI H7N9 viruses were isolated from duck meat products carried illegally and relinquished voluntarily at the border by passengers on flights from China to Japan between 2016 and 2017. Some of the LPAI and HPAI H7N9 viruses detected at the border in Japan were characterized previously in chickens and ducks; however, their pathogenicity and replicative ability in mammals remain unknown. In this study, we assessed the biological features of two HPAI H7N9 virus isolates [A/duck/Japan/AQ-HE29-22/2017 (HE29-22) and A/duck/Japan/AQ-HE29-52/2017 (HE29-52); both of these viruses were isolated from duck meat at the border)] and an LPAI H7N9 virus isolate [A/duck/Japan/AQ-HE28-3/2016 (HE28-3)] in mice and ferrets. In mice, HE29-52 was more pathogenic than HE29-22 and HE28-3. In ferrets, the two HPAI virus isolates replicated more efficiently in the lower respiratory tract of the animals than did the LPAI virus isolate. Our results indicate that HPAI H7N9 viruses with potential to cause severe diseases in mammals have been illegally introduced to Japan.
? 2019 Blackwell Verlag GmbH.
[h=4]KEYWORDS:[/h] H7N9; Highly pathogenic avian influenza; pathogenicity in mammals
PMID: 31650680 DOI: 10.1111/tbed.13398