tetano
Editor, Senior Moderator
J Virol. 2012 Oct 10. [Epub ahead of print]
Cellular Protein HAX1 Interacts With Influenza A Virus Polymerase PA Subunit and Impedes Its Nuclear Translocation.
Hsu WB, Shih JL, Shih JR, Du JL, Teng SC, Huang LM, Wang WB.
Source
Graduate Institute of Microbiology, College of Medicine.
Abstract
Transcription and replication of the influenza A virus RNA genome occurs in the nucleus through the viral RNA-dependent RNA polymerase consisting of PB1, PB2, and PA. Cellular factors that associate with the viral polymerase complex play important roles in these processes. To look for cellular factors that could associate with influenza A virus PA protein, we have carried out a yeast two-hybrid screen using a HeLa cell cDNA library. We identified six cellular proteins that may interact with PA. In this report, we focused our study on one of the new PA-interacting proteins HAX1, a protein with anti-apoptotic function. By using Glutathione S-transferase pull-down and coimmunoprecipitation assays, we demonstrated that HAX1 specifically interacted with PA in vitro and in vivo, and that HAX1 interacted with the nuclear localization signal domain of PA. Nuclear accumulation of PA was increased in HAX1-knockdown cells and this phenotype could be reversed by reexpression of HAX1, indicating that HAX1 can impede nuclear transport of PA. As a consequence, knockdown of HAX1 resulted in a significant increase on virus yield and polymerase activity in a minigenome assay and this phenotype could be reversed by reexpression of HAX1, indicating that HAX1 can inhibit influenza A virus propagation. Together, these results not only provide an insight about the mechanism underlying nuclear transport of PA but also identify an intrinsic host factor that restricts influenza A virus infection.
PMID:
23055567
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23055567
Cellular Protein HAX1 Interacts With Influenza A Virus Polymerase PA Subunit and Impedes Its Nuclear Translocation.
Hsu WB, Shih JL, Shih JR, Du JL, Teng SC, Huang LM, Wang WB.
Source
Graduate Institute of Microbiology, College of Medicine.
Abstract
Transcription and replication of the influenza A virus RNA genome occurs in the nucleus through the viral RNA-dependent RNA polymerase consisting of PB1, PB2, and PA. Cellular factors that associate with the viral polymerase complex play important roles in these processes. To look for cellular factors that could associate with influenza A virus PA protein, we have carried out a yeast two-hybrid screen using a HeLa cell cDNA library. We identified six cellular proteins that may interact with PA. In this report, we focused our study on one of the new PA-interacting proteins HAX1, a protein with anti-apoptotic function. By using Glutathione S-transferase pull-down and coimmunoprecipitation assays, we demonstrated that HAX1 specifically interacted with PA in vitro and in vivo, and that HAX1 interacted with the nuclear localization signal domain of PA. Nuclear accumulation of PA was increased in HAX1-knockdown cells and this phenotype could be reversed by reexpression of HAX1, indicating that HAX1 can impede nuclear transport of PA. As a consequence, knockdown of HAX1 resulted in a significant increase on virus yield and polymerase activity in a minigenome assay and this phenotype could be reversed by reexpression of HAX1, indicating that HAX1 can inhibit influenza A virus propagation. Together, these results not only provide an insight about the mechanism underlying nuclear transport of PA but also identify an intrinsic host factor that restricts influenza A virus infection.
PMID:
23055567
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23055567