tetano
Editor, Senior Moderator
Cell Rep Med
. 2021 Jan 16;100193.
doi: 10.1016/j.xcrm.2021.100193. Online ahead of print.
Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic COVID-19 patients
Yun Shan Goh[SUP] 1 2 [/SUP], Jean-Marc Chavatte[SUP] 3 [/SUP], Alicia Lim Jieling[SUP] 3 [/SUP], Bernett Lee[SUP] 2 [/SUP], Pei Xiang Hor[SUP] 1 2 [/SUP], Siti Naqiah Amrun[SUP] 1 2 [/SUP], Cheryl Yi-Pin Lee[SUP] 1 2 [/SUP], Rhonda Sin-Ling Chee[SUP] 1 2 [/SUP], Bei Wang[SUP] 2 [/SUP], Chia Yin Lee[SUP] 2 [/SUP], Eve Zhi Xian Ngoh[SUP] 2 [/SUP], Cheng-I Wang[SUP] 2 [/SUP], Barnaby Edward Young[SUP] 3 4 5 [/SUP], Paul A Tambyah[SUP] 3 6 [/SUP], Shirin Kalimuddin[SUP] 7 8 [/SUP], Surinder Pada[SUP] 9 [/SUP], Seow-Yen Tan[SUP] 10 [/SUP], Louisa Jin Sun[SUP] 11 [/SUP], Mark I-Cheng Chen[SUP] 3 12 [/SUP], Yee-Sin Leo[SUP] 3 4 5 13 [/SUP], David C Lye[SUP] 3 4 5 13 [/SUP], Lisa F P Ng[SUP] 1 2 14 15 16 17 [/SUP], Raymond Tzer Pin Lin[SUP] 3 13 [/SUP], Laurent Renia[SUP] 1 2 17 [/SUP]
Affiliations
Abstract
Early detection of infections is crucial to limit the spread of coronavirus 2019 disease (COVID-19). Here, we develop a flow cytometry-based assay to detect SARS-CoV-2 Spike protein (S protein) antibodies in COVID-19 patients. The assay detects specific IgM, IgA and IgG in COVID-19 patients and also the acquisition of all IgG subclasses, with IgG1 being the most dominant. The antibody response is significantly higher at a later stage of the infection. Furthermore, asymptomatic COVID-19 patients also develop specific IgM, IgA and IgG, with IgG1 as the most dominant subclass. Although the antibody levels are lower in asymptomatic infections, the assay is highly sensitive and detect 97% of asymptomatic infections. These findings demonstrate that the assay can be used for serological analysis of symptomatic infections, and also asymptomatic infections, which may, otherwise, go undetected.
Keywords: COVID-19; IgG subclasses; S protein; SARS-CoV-2; antibodies; asymptomatic; serological; symptomatic.
. 2021 Jan 16;100193.
doi: 10.1016/j.xcrm.2021.100193. Online ahead of print.
Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic COVID-19 patients
Yun Shan Goh[SUP] 1 2 [/SUP], Jean-Marc Chavatte[SUP] 3 [/SUP], Alicia Lim Jieling[SUP] 3 [/SUP], Bernett Lee[SUP] 2 [/SUP], Pei Xiang Hor[SUP] 1 2 [/SUP], Siti Naqiah Amrun[SUP] 1 2 [/SUP], Cheryl Yi-Pin Lee[SUP] 1 2 [/SUP], Rhonda Sin-Ling Chee[SUP] 1 2 [/SUP], Bei Wang[SUP] 2 [/SUP], Chia Yin Lee[SUP] 2 [/SUP], Eve Zhi Xian Ngoh[SUP] 2 [/SUP], Cheng-I Wang[SUP] 2 [/SUP], Barnaby Edward Young[SUP] 3 4 5 [/SUP], Paul A Tambyah[SUP] 3 6 [/SUP], Shirin Kalimuddin[SUP] 7 8 [/SUP], Surinder Pada[SUP] 9 [/SUP], Seow-Yen Tan[SUP] 10 [/SUP], Louisa Jin Sun[SUP] 11 [/SUP], Mark I-Cheng Chen[SUP] 3 12 [/SUP], Yee-Sin Leo[SUP] 3 4 5 13 [/SUP], David C Lye[SUP] 3 4 5 13 [/SUP], Lisa F P Ng[SUP] 1 2 14 15 16 17 [/SUP], Raymond Tzer Pin Lin[SUP] 3 13 [/SUP], Laurent Renia[SUP] 1 2 17 [/SUP]
Affiliations
- PMID: 33495757
- PMCID: PMC7816583
- DOI: 10.1016/j.xcrm.2021.100193
Abstract
Early detection of infections is crucial to limit the spread of coronavirus 2019 disease (COVID-19). Here, we develop a flow cytometry-based assay to detect SARS-CoV-2 Spike protein (S protein) antibodies in COVID-19 patients. The assay detects specific IgM, IgA and IgG in COVID-19 patients and also the acquisition of all IgG subclasses, with IgG1 being the most dominant. The antibody response is significantly higher at a later stage of the infection. Furthermore, asymptomatic COVID-19 patients also develop specific IgM, IgA and IgG, with IgG1 as the most dominant subclass. Although the antibody levels are lower in asymptomatic infections, the assay is highly sensitive and detect 97% of asymptomatic infections. These findings demonstrate that the assay can be used for serological analysis of symptomatic infections, and also asymptomatic infections, which may, otherwise, go undetected.
Keywords: COVID-19; IgG subclasses; S protein; SARS-CoV-2; antibodies; asymptomatic; serological; symptomatic.