tetano
Editor, Senior Moderator
Eur J Immunol. 2013 Sep 30. doi: 10.1002/eji.201343583. [Epub ahead of print]
CD8+ Treg cells suppress CD8+ T cell-responses by IL-10-dependent mechanism during H5N1 influenza virus infection.
Zou Q, Wu B, Xue J, Fan X, Feng C, Geng S, Wang M, Wang B.
Source
Key laboratory of Medical Molecular Virology of MOH and MOE, Fudan University Shanghai Medical College, Shanghai, China; State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing, China; Present Address: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
Abstract
Although Treg-cell mediated suppression during infection or autoimmunity has been described, functions of Treg cells during highly pathogenic avian influenza virus infection remain poorly characterized. Here we found that in Foxp3-GFP transgenic mice, CD8+ Foxp3+ Treg cells, but not CD4+ Foxp3+ Treg cells, were remarkably induced during H5N1 infection. In addition to expressing CD25, the CD8+ Foxp3+ Treg cells showed a high level of GITR and produced IL-10. In an adoptive transfer model, CD8+ Treg cells suppressed CD8+ T cell-responses and promoted H5N1 virus infection, resulting in enhanced mortality and increased virus load in the lung. Furthermore, in vitro neutralization of IL-10 and studies with IL-10R-deficient mice in vitro and in vivo demonstrated an important role for IL-10 production in the capacity of CD8+ Treg cells to inhibit CD8+ T cell-responses. Our findings identify a previously unrecognized role of CD8+ Treg cells in the negative regulation of CD8+ T cell-responses and suggest that modulation of CD8+ Treg cells may be a therapeutic strategy to control H5N1 viral infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
CD8+ Treg cells, H5N1 influenza virus, IL-10
PMID:
24114149
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24114149
CD8+ Treg cells suppress CD8+ T cell-responses by IL-10-dependent mechanism during H5N1 influenza virus infection.
Zou Q, Wu B, Xue J, Fan X, Feng C, Geng S, Wang M, Wang B.
Source
Key laboratory of Medical Molecular Virology of MOH and MOE, Fudan University Shanghai Medical College, Shanghai, China; State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing, China; Present Address: Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA.
Abstract
Although Treg-cell mediated suppression during infection or autoimmunity has been described, functions of Treg cells during highly pathogenic avian influenza virus infection remain poorly characterized. Here we found that in Foxp3-GFP transgenic mice, CD8+ Foxp3+ Treg cells, but not CD4+ Foxp3+ Treg cells, were remarkably induced during H5N1 infection. In addition to expressing CD25, the CD8+ Foxp3+ Treg cells showed a high level of GITR and produced IL-10. In an adoptive transfer model, CD8+ Treg cells suppressed CD8+ T cell-responses and promoted H5N1 virus infection, resulting in enhanced mortality and increased virus load in the lung. Furthermore, in vitro neutralization of IL-10 and studies with IL-10R-deficient mice in vitro and in vivo demonstrated an important role for IL-10 production in the capacity of CD8+ Treg cells to inhibit CD8+ T cell-responses. Our findings identify a previously unrecognized role of CD8+ Treg cells in the negative regulation of CD8+ T cell-responses and suggest that modulation of CD8+ Treg cells may be a therapeutic strategy to control H5N1 viral infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
CD8+ Treg cells, H5N1 influenza virus, IL-10
PMID:
24114149
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24114149