tetano
Editor, Senior Moderator
Immunol Cell Biol. 2014 Apr 29. doi: 10.1038/icb.2014.28. [Epub ahead of print]
CD154+ CD4+ T-cell dependence for effective memory influenza virus-specific CD8+ T-cell responses.
Olson MR1, Seah SG2, Edenborough K1, Doherty PC3, Lew AM2, Turner SJ1.
Author information
Abstract
CD40-CD154 (CD40 ligand) interactions are essential for the efficient priming of CD8+ cytotoxic T lymphocyte (CTL) responses. This is typically via CD4+CD154+ T-cell-dependent 'licensing' of CD40+ dendritic cells (DCs); however, DCs infected with influenza A virus (IAV) upregulate CD154 expression, thus enabling efficient CTL priming in the absence of CD4+ T activation. Therefore, it is unclear whether CD4 T cells and DCs have redundant or unique roles in the priming of primary and secondary CTL responses after infection. Here we determine the precise cellular interactions involved in CD40-CD154 regulation of both primary and secondary IAV-specific CTL responses. Infection of both CD40 KO and CD154 KO mice resulted in diminished quantitative and qualitative CTL responses after both primary and secondary infection. Adoptive transfer of CD154+, but not CD154 KO, CD4 T cells into CD154 KO mice restored both primary and secondary IAV-specific CD8 T-cell responses. These data show that, although CD154 expression on CD4 T cells and other cell types (that is, DCs) may be redundant for the priming of primary CTL responses, CD154 expression by CD4 T cells is required for the priming memory CD8 T cells that are capable of fully responding to secondary infection.Immunology and Cell Biology advance online publication, 29 April 2014; doi:10.1038/icb.2014.28.
PMID:
24777309
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24777309
CD154+ CD4+ T-cell dependence for effective memory influenza virus-specific CD8+ T-cell responses.
Olson MR1, Seah SG2, Edenborough K1, Doherty PC3, Lew AM2, Turner SJ1.
Author information
Abstract
CD40-CD154 (CD40 ligand) interactions are essential for the efficient priming of CD8+ cytotoxic T lymphocyte (CTL) responses. This is typically via CD4+CD154+ T-cell-dependent 'licensing' of CD40+ dendritic cells (DCs); however, DCs infected with influenza A virus (IAV) upregulate CD154 expression, thus enabling efficient CTL priming in the absence of CD4+ T activation. Therefore, it is unclear whether CD4 T cells and DCs have redundant or unique roles in the priming of primary and secondary CTL responses after infection. Here we determine the precise cellular interactions involved in CD40-CD154 regulation of both primary and secondary IAV-specific CTL responses. Infection of both CD40 KO and CD154 KO mice resulted in diminished quantitative and qualitative CTL responses after both primary and secondary infection. Adoptive transfer of CD154+, but not CD154 KO, CD4 T cells into CD154 KO mice restored both primary and secondary IAV-specific CD8 T-cell responses. These data show that, although CD154 expression on CD4 T cells and other cell types (that is, DCs) may be redundant for the priming of primary CTL responses, CD154 expression by CD4 T cells is required for the priming memory CD8 T cells that are capable of fully responding to secondary infection.Immunology and Cell Biology advance online publication, 29 April 2014; doi:10.1038/icb.2014.28.
PMID:
24777309
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24777309