tetano
Editor, Senior Moderator
Int Immunol. 2019 Aug 1. pii: dxz054. doi: 10.1093/intimm/dxz054. [Epub ahead of print]
[h=1]Broad and systemic immune-modulating capacity of plant-derived dsRNA.[/h] Hajake T[SUP]1,[/SUP][SUP]2[/SUP], Matsuno K[SUP]3[/SUP], M Kasumba D[SUP]1,[/SUP][SUP]2[/SUP], Oda H[SUP]1,[/SUP][SUP]2[/SUP], Kobayashi M[SUP]1[/SUP], Miyata N[SUP]1,[/SUP][SUP]2[/SUP], Shinji M[SUP]1,[/SUP][SUP]2[/SUP], Kogure A[SUP]1,[/SUP][SUP]2[/SUP], Kasajima N[SUP]3[/SUP], Okamatsu M[SUP]4[/SUP], Sakoda Y[SUP]3,[/SUP][SUP]4[/SUP], Kato H[SUP]1,[/SUP][SUP]2,[/SUP][SUP]5[/SUP], Fujita T[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Double-stranded RNA (dsRNA) is well characterized as an inducer of antiviral interferon responses. We previously reported that dsRNA extracted from a specific edible plant possesses immune-modulating capacity to confer mice the resistance against respiratory viruses, including the H1N1 strain of the Influenza A virus. We report that the systemic immune-activating capacity of the plant-derived dsRNA protected mice from infection by a highly virulent H5N1 strain of the Influenza A virus. In addition, subcutaneous inoculation of the dsRNA together with inactivated virion of the H5N1 strain of the Influenza A virus suppressed the lethality by the viral infection as compared with individual inoculation of either dsRNA or HA protein, suggesting its potential usage as a vaccination adjuvant. Moreover, intraperitoneal inoculation of the dsRNA limited the growth of B16-F10 melanoma cells through the activation of NK cells in murine models. Taken together, this study demonstrated the systemic immune-modulating capacity of a plant-derived dsRNA and its potential for nucleic acid-based clinical applications.
? The Japanese Society for Immunology. 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
[h=4]KEYWORDS:[/h] adjuvant; antiviral; double-stranded RNA; melanoma; type I interferon
PMID: 31367737 DOI: 10.1093/intimm/dxz054
[h=1]Broad and systemic immune-modulating capacity of plant-derived dsRNA.[/h] Hajake T[SUP]1,[/SUP][SUP]2[/SUP], Matsuno K[SUP]3[/SUP], M Kasumba D[SUP]1,[/SUP][SUP]2[/SUP], Oda H[SUP]1,[/SUP][SUP]2[/SUP], Kobayashi M[SUP]1[/SUP], Miyata N[SUP]1,[/SUP][SUP]2[/SUP], Shinji M[SUP]1,[/SUP][SUP]2[/SUP], Kogure A[SUP]1,[/SUP][SUP]2[/SUP], Kasajima N[SUP]3[/SUP], Okamatsu M[SUP]4[/SUP], Sakoda Y[SUP]3,[/SUP][SUP]4[/SUP], Kato H[SUP]1,[/SUP][SUP]2,[/SUP][SUP]5[/SUP], Fujita T[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Double-stranded RNA (dsRNA) is well characterized as an inducer of antiviral interferon responses. We previously reported that dsRNA extracted from a specific edible plant possesses immune-modulating capacity to confer mice the resistance against respiratory viruses, including the H1N1 strain of the Influenza A virus. We report that the systemic immune-activating capacity of the plant-derived dsRNA protected mice from infection by a highly virulent H5N1 strain of the Influenza A virus. In addition, subcutaneous inoculation of the dsRNA together with inactivated virion of the H5N1 strain of the Influenza A virus suppressed the lethality by the viral infection as compared with individual inoculation of either dsRNA or HA protein, suggesting its potential usage as a vaccination adjuvant. Moreover, intraperitoneal inoculation of the dsRNA limited the growth of B16-F10 melanoma cells through the activation of NK cells in murine models. Taken together, this study demonstrated the systemic immune-modulating capacity of a plant-derived dsRNA and its potential for nucleic acid-based clinical applications.
? The Japanese Society for Immunology. 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
[h=4]KEYWORDS:[/h] adjuvant; antiviral; double-stranded RNA; melanoma; type I interferon
PMID: 31367737 DOI: 10.1093/intimm/dxz054