tetano
Editor, Senior Moderator
BMC Infect Dis
. 2024 Jul 9;24(1):679.
doi: 10.1186/s12879-024-09574-9. Evaluation and comparison of one-step real-time PCR and one-step RT-LAMP methods for detection of SARS-CoV-2
Hooman Hanifehpour[SUP] 1 [/SUP], Fatemeh Ashrafi[SUP] 1 [/SUP], Elham Siasi[SUP] 1 [/SUP], Shirzad Fallahi[SUP] 2 3 [/SUP]
Affiliations
Background: There is an increasing disease trend for SARS-COV-2, so need a quick and affordable diagnostic method. It should be highly accurate and save costs compared to other methods. The purpose of this research is to achieve these goals.
Methods: This study analyzed 342 samples using TaqMan One-Step RT-qPCR and fast One-Step RT-LAMP (Reverse Transcriptase Loop-Mediated Isothermal Amplification). The One-Step LAMP assay was conducted to assess the sensitivity and specificity.
Results: The research reported positive samples using two different methods. In the RT-LAMP method, saliva had 92 positive samples (26.9%) and 250 negative samples (73.09%) and nasopharynx had 94 positive samples (27.4%) and 248 negative samples (72.51%). In the RT-qPCR method, saliva had 86 positive samples (25.1%) and 256 negative samples (74.8%) and nasopharynx had 93 positive samples (27.1%) and 249 negative samples (72.8%). The agreement between the two tests in saliva and nasopharynx samples was 93% and 94% respectively, based on Cohen's kappa coefficient (κ) (P < 0.001). The rate of sensitivity in this technique was reported at a dilution of 1 × 10[SUP]1[/SUP] and 100% specificity.
Conclusions: Based on the results of the study the One-Step LAMP assay has multiple advantages. These include simplicity, cost-effectiveness, high sensitivity, and specificity. The One-Step LAMP assay shows promise as a diagnostic tool. It can help manage disease outbreaks, ensure prompt treatment, and safeguard public health by providing rapid, easy-to-use testing.
Keywords: Detection; One-step LAMP; One-step RT-qPCR; Rapid; SARS-CoV-2; Sensitive.
. 2024 Jul 9;24(1):679.
doi: 10.1186/s12879-024-09574-9. Evaluation and comparison of one-step real-time PCR and one-step RT-LAMP methods for detection of SARS-CoV-2
Hooman Hanifehpour[SUP] 1 [/SUP], Fatemeh Ashrafi[SUP] 1 [/SUP], Elham Siasi[SUP] 1 [/SUP], Shirzad Fallahi[SUP] 2 3 [/SUP]
Affiliations
- PMID: 38982392
- PMCID: PMC11232332
- DOI: 10.1186/s12879-024-09574-9
Background: There is an increasing disease trend for SARS-COV-2, so need a quick and affordable diagnostic method. It should be highly accurate and save costs compared to other methods. The purpose of this research is to achieve these goals.
Methods: This study analyzed 342 samples using TaqMan One-Step RT-qPCR and fast One-Step RT-LAMP (Reverse Transcriptase Loop-Mediated Isothermal Amplification). The One-Step LAMP assay was conducted to assess the sensitivity and specificity.
Results: The research reported positive samples using two different methods. In the RT-LAMP method, saliva had 92 positive samples (26.9%) and 250 negative samples (73.09%) and nasopharynx had 94 positive samples (27.4%) and 248 negative samples (72.51%). In the RT-qPCR method, saliva had 86 positive samples (25.1%) and 256 negative samples (74.8%) and nasopharynx had 93 positive samples (27.1%) and 249 negative samples (72.8%). The agreement between the two tests in saliva and nasopharynx samples was 93% and 94% respectively, based on Cohen's kappa coefficient (κ) (P < 0.001). The rate of sensitivity in this technique was reported at a dilution of 1 × 10[SUP]1[/SUP] and 100% specificity.
Conclusions: Based on the results of the study the One-Step LAMP assay has multiple advantages. These include simplicity, cost-effectiveness, high sensitivity, and specificity. The One-Step LAMP assay shows promise as a diagnostic tool. It can help manage disease outbreaks, ensure prompt treatment, and safeguard public health by providing rapid, easy-to-use testing.
Keywords: Detection; One-step LAMP; One-step RT-qPCR; Rapid; SARS-CoV-2; Sensitive.