Giuseppe
Emeritus
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs (BMC, abstract, edited)
[Source Full PDF Document: LINK. EDITED.]
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs
Virology Journal 2010, 7:23 doi:10.1186/1743-422X-7-23
Sara B Hamilton (shamilton@mriresearch.org) - Deirdre E Daniels (ddaniels@mriresearch.org) - William A Sosna (wsosna@mriresearch.org) - Eric R Jeppesen (ejeppesen@mriresearch.org) - Julie M Owells (jowells@mriresearch.org) - Micah D Halpern (mhalpern@mriresearch.org) - Kimberly S McCurdy (kmccurdy@mriresearch.org) - Jonathan O Rayner (jrayner@mriresearch.org) - John A Lednicky (jlednicky@mriresearch.org)
ISSN 1743-422X
Article type Methodology
Submission date 8 January 2010
Acceptance date 28 January 2010
Publication date 28 January 2010
Article URL (LINK)
This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below).
Articles in Virology Journal are listed in PubMed and archived at PubMed Central.
For information about publishing your research in Virology Journal or any BioMed Central journal, go to (LINK)
For information about other BioMed Central publications go to Virology Journal
? 2010 Hamilton et al. , licensee BioMed Central Ltd.
This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs
Sara B Hamilton1, Deirdre E Daniels1, William A Sosna1, Eric R Jeppesen2, Julie M Owells1, Micah D Halpern1, Kimberly S McCurdy1, Jonathan O Rayner1, and John A Lednicky 1?
1Energy and Life Sciences Division, Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri, 64110, United States of America
2Biosafety/Biosurety Office, Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri, 64110, United States of America
?Corresponding author
Email addresses:
SBH: shamilton@mriresearch.org; DED: ddaniels@mriresearch.org; WAS:
wsosna@mriresearch.org; ERJ: ejeppesen@mriresearch.org; JMO:
jowells@mriresearch.org; MDH: mhalpern@mriresearch.org; KSM:
kmccurdy@mriresearch.org; JOR: jrayner@mriresearch.org; JAL:
jlednicky@mriresearch.org
Abstract
Background
Embryonated chicken eggs (ECE) are sometimes used for the primary isolation or passage of influenza viruses, other viruses, and certain bacteria. For small-scale experiments with pathogens that must be studied in biosafety level three (BSL3) facilities, inoculated ECE are sometimes manipulated and maintained in small egg incubators within a biosafety cabinet (BSC). To simplify the clean up and decontamination of an egg incubator in case of egg breakage, we explored whether ethylene breather bags could be used to encase ECE inoculated with pathogens. This concept was tested by determining embryo survival and examining virus yields in bagged ECE.
Results
Virus yields acceptable for many applications were attained when influenza-, alpha-, flavi-, canine distemper-, and mousepox viruses were propagated in ECE sealed within ethylene breather bags.
Conclusions
For many small-scale applications, ethylene breather bags can be used to encase ECE inoculated with various viruses.
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[Source Full PDF Document: LINK. EDITED.]
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs
Virology Journal 2010, 7:23 doi:10.1186/1743-422X-7-23
Sara B Hamilton (shamilton@mriresearch.org) - Deirdre E Daniels (ddaniels@mriresearch.org) - William A Sosna (wsosna@mriresearch.org) - Eric R Jeppesen (ejeppesen@mriresearch.org) - Julie M Owells (jowells@mriresearch.org) - Micah D Halpern (mhalpern@mriresearch.org) - Kimberly S McCurdy (kmccurdy@mriresearch.org) - Jonathan O Rayner (jrayner@mriresearch.org) - John A Lednicky (jlednicky@mriresearch.org)
ISSN 1743-422X
Article type Methodology
Submission date 8 January 2010
Acceptance date 28 January 2010
Publication date 28 January 2010
Article URL (LINK)
This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below).
Articles in Virology Journal are listed in PubMed and archived at PubMed Central.
For information about publishing your research in Virology Journal or any BioMed Central journal, go to (LINK)
For information about other BioMed Central publications go to Virology Journal
? 2010 Hamilton et al. , licensee BioMed Central Ltd.
This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs
Sara B Hamilton1, Deirdre E Daniels1, William A Sosna1, Eric R Jeppesen2, Julie M Owells1, Micah D Halpern1, Kimberly S McCurdy1, Jonathan O Rayner1, and John A Lednicky 1?
1Energy and Life Sciences Division, Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri, 64110, United States of America
2Biosafety/Biosurety Office, Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri, 64110, United States of America
?Corresponding author
Email addresses:
SBH: shamilton@mriresearch.org; DED: ddaniels@mriresearch.org; WAS:
wsosna@mriresearch.org; ERJ: ejeppesen@mriresearch.org; JMO:
jowells@mriresearch.org; MDH: mhalpern@mriresearch.org; KSM:
kmccurdy@mriresearch.org; JOR: jrayner@mriresearch.org; JAL:
jlednicky@mriresearch.org
Abstract
Background
Embryonated chicken eggs (ECE) are sometimes used for the primary isolation or passage of influenza viruses, other viruses, and certain bacteria. For small-scale experiments with pathogens that must be studied in biosafety level three (BSL3) facilities, inoculated ECE are sometimes manipulated and maintained in small egg incubators within a biosafety cabinet (BSC). To simplify the clean up and decontamination of an egg incubator in case of egg breakage, we explored whether ethylene breather bags could be used to encase ECE inoculated with pathogens. This concept was tested by determining embryo survival and examining virus yields in bagged ECE.
Results
Virus yields acceptable for many applications were attained when influenza-, alpha-, flavi-, canine distemper-, and mousepox viruses were propagated in ECE sealed within ethylene breather bags.
Conclusions
For many small-scale applications, ethylene breather bags can be used to encase ECE inoculated with various viruses.
-
------