tetano
Editor, Senior Moderator
Chem Asian J. 2015 Mar 29. doi: 10.1002/asia.201500105. [Epub ahead of print]
[h=1]Biometallization-based electrochemical magnetoimmunosensing strategy for avian influenza A (H7N9) virus particle detection.[/h] Zhou CH[SUP]1[/SUP], Wu Z, Chen JJ, Xiong C, Chen Z, Pang DW, Zhang ZL.
[h=3]Author information[/h]
[h=3]Abstract[/h] A highly sensitive electrochemical immunosensor for avian influenza A (H7N9) virus (H7N9 AIV) detection was proposed by using electrochemical magnetoimmunoassay coupled with biometallization and anodic stripping voltammetry. This strategy could accumulate the enzyme-generated product on the surface of the magneto electrode by means of silver deposition, which amplified the detection signal in about 80 times. The use of magnetic bead (MB) and the magneto electrode could also amplify the detection signal. Furthermore, a bi-electrode signal transduction system was introduced into this immunosensor, which also beneficial to the immunoassay. As low as 0.011 ng mL-1 H7N9 AIV could be detected in about 1.5 h with good specificity and strong anti-interference ability. This study not only provides a simple and sensitive approach for virus detection, but also offers an effective signal enhancement strategy for the development of highly sensitive MB-based electrochemical immunoassay method.
Copyright ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] Avian influenza A (H7N9) virus; bi-electrode signal transduction; biometallization; electrochemical immunosensor; magnetic bead
PMID: 25820789 [PubMed - as supplied by publisher]
[h=1]Biometallization-based electrochemical magnetoimmunosensing strategy for avian influenza A (H7N9) virus particle detection.[/h] Zhou CH[SUP]1[/SUP], Wu Z, Chen JJ, Xiong C, Chen Z, Pang DW, Zhang ZL.
[h=3]Author information[/h]
[h=3]Abstract[/h] A highly sensitive electrochemical immunosensor for avian influenza A (H7N9) virus (H7N9 AIV) detection was proposed by using electrochemical magnetoimmunoassay coupled with biometallization and anodic stripping voltammetry. This strategy could accumulate the enzyme-generated product on the surface of the magneto electrode by means of silver deposition, which amplified the detection signal in about 80 times. The use of magnetic bead (MB) and the magneto electrode could also amplify the detection signal. Furthermore, a bi-electrode signal transduction system was introduced into this immunosensor, which also beneficial to the immunoassay. As low as 0.011 ng mL-1 H7N9 AIV could be detected in about 1.5 h with good specificity and strong anti-interference ability. This study not only provides a simple and sensitive approach for virus detection, but also offers an effective signal enhancement strategy for the development of highly sensitive MB-based electrochemical immunoassay method.
Copyright ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] Avian influenza A (H7N9) virus; bi-electrode signal transduction; biometallization; electrochemical immunosensor; magnetic bead
PMID: 25820789 [PubMed - as supplied by publisher]