tetano
Editor, Senior Moderator
Bioengineered
. 2022 Feb;13(2):2486-2497.
doi: 10.1080/21655979.2021.2014621.
Inflammatory activation and immune cell infiltration are main biological characteristics of SARS-CoV-2 infected myocardium
Rui Zhang[SUP] 1 [/SUP], Xi Chen[SUP] 1 [/SUP], Wenjie Zuo[SUP] 1 [/SUP], Zhenjun Ji[SUP] 1 [/SUP], Yangyang Qu[SUP] 1 [/SUP], Yamin Su[SUP] 1 [/SUP], Mingming Yang[SUP] 1 [/SUP], Pengfei Zuo[SUP] 1 [/SUP], Genshan Ma[SUP] 1 [/SUP], Yongjun Li[SUP] 1 [/SUP]
Affiliations
Abstract
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can target cardiomyocytes (CMs) to directly invade the heart resulting in high mortality. This study aims to explore the biological characteristics of SARS-CoV-2 infected myocardium based on omics by collecting transcriptome data and analyzing them with a series of bioinformatics tools. Totally, 86 differentially expressed genes (DEGs) were discovered in SARS-CoV-2 infected CMs, and 15 miRNAs were discovered to target 60 genes. Functional enrichment analysis indicated that these DEGs were mainly enriched in the inflammatory signaling pathway. After the protein-protein interaction (PPI) network was constructed, several genes including CCL2 and CXCL8 were regarded as the hub genes. SRC inhibitor saracatinib was predicted to potentially act against the cardiac dysfunction induced by SARS-CoV-2. Among the 86 DEGs, 28 were validated to be dysregulated in SARS-CoV-2 infected hearts. Gene Set Enrichment Analysis (GSEA) analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that malaria, IL-17 signaling pathway, and complement and coagulation cascades were significantly enriched. Immune infiltration analysis indicated that 'naive B cells' was significantly increased in the SARS-CoV-2 infected heart. The above results may help to improve the prognosis of patients with COVID-19.
Keywords: COVID-19; SARS-CoV-2; bioinformatics; cardiomyocyte; heart injury.
. 2022 Feb;13(2):2486-2497.
doi: 10.1080/21655979.2021.2014621.
Inflammatory activation and immune cell infiltration are main biological characteristics of SARS-CoV-2 infected myocardium
Rui Zhang[SUP] 1 [/SUP], Xi Chen[SUP] 1 [/SUP], Wenjie Zuo[SUP] 1 [/SUP], Zhenjun Ji[SUP] 1 [/SUP], Yangyang Qu[SUP] 1 [/SUP], Yamin Su[SUP] 1 [/SUP], Mingming Yang[SUP] 1 [/SUP], Pengfei Zuo[SUP] 1 [/SUP], Genshan Ma[SUP] 1 [/SUP], Yongjun Li[SUP] 1 [/SUP]
Affiliations
- PMID: 35037831
- DOI: 10.1080/21655979.2021.2014621
Abstract
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can target cardiomyocytes (CMs) to directly invade the heart resulting in high mortality. This study aims to explore the biological characteristics of SARS-CoV-2 infected myocardium based on omics by collecting transcriptome data and analyzing them with a series of bioinformatics tools. Totally, 86 differentially expressed genes (DEGs) were discovered in SARS-CoV-2 infected CMs, and 15 miRNAs were discovered to target 60 genes. Functional enrichment analysis indicated that these DEGs were mainly enriched in the inflammatory signaling pathway. After the protein-protein interaction (PPI) network was constructed, several genes including CCL2 and CXCL8 were regarded as the hub genes. SRC inhibitor saracatinib was predicted to potentially act against the cardiac dysfunction induced by SARS-CoV-2. Among the 86 DEGs, 28 were validated to be dysregulated in SARS-CoV-2 infected hearts. Gene Set Enrichment Analysis (GSEA) analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that malaria, IL-17 signaling pathway, and complement and coagulation cascades were significantly enriched. Immune infiltration analysis indicated that 'naive B cells' was significantly increased in the SARS-CoV-2 infected heart. The above results may help to improve the prognosis of patients with COVID-19.
Keywords: COVID-19; SARS-CoV-2; bioinformatics; cardiomyocyte; heart injury.