Biochip J . Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

[tetano]

Biochip J


. 2021 Feb 17;1-9.
doi: 10.1007/s13206-021-00011-6. Online ahead of print.
Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies


Ji-Hong Bong[SUP] 1 [/SUP], Tae-Hun Kim[SUP] 1 [/SUP], Jaeyong Jung[SUP] 1 [/SUP], Soo Jeong Lee[SUP] 1 [/SUP], Jeong Soo Sung[SUP] 1 [/SUP], Chang Kyu Lee[SUP] 1 [/SUP], Min-Jung Kang[SUP] 2 [/SUP], Hyun Ok Kim[SUP] 3 [/SUP], Jae-Chul Pyun[SUP] 1 [/SUP]



Affiliations

• PMID: 33613853
• PMCID: PMC7887419
• DOI: 10.1007/s13206-021-00011-6

Abstract

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

Keywords: Anti-SARS-CoV-2 nucleoprotein antibody; Competitive assay; Culture fluid; Nucleoprotein (NP); SARS-CoV-2.