tetano
Editor, Senior Moderator
J Chromatogr A. 2013 Dec 19. pii: S0021-9673(13)01873-6. doi: 10.1016/j.chroma.2013.12.009. [Epub ahead of print]
Avidity of influenza virus: Model-based identification of adsorption kinetics from surface plasmon resonance experiments.
Wang W1, Wolff MW2, Reichl U2, Sundmacher K3.
Author information
Abstract
Affinity chromatography and membrane adsorption are highly promising methods for the downstream processing of cell culture-derived influenza virus. For the optimization of this separation process, it is desirable to quantify the kinetics of virus adsorption. For this reason, the adsorption kinetics of the influenza A virus (Puerto Rico/8/34 (H1N1)) on a surface with the immobilized ligand Euronymus europaeus lectin (EEL) was investigated. The adsorption kinetics was experimentally monitored in a microfluidic flow cell by surface plasmon resonance (SPR) spectroscopy. The boundary layer theory was applied to analyze the convective and diffusive mass transport of the virus particles in the SPR flow cell. A multi-site kinetic adsorption model was found to describe the experimentally recorded adsorption curves adequately. According to the proposed model, under the applied experimental conditions, the number of sites (galactose residuals) binding one single virus particle to the EEL surface is in the range of 300 to 460, which is in average about 4% of the total number of sites available on the virus surface. The avidity of individual virus particles to the EEL surface was estimated to be in the order of magnitude of 106M-1s-1.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Adsorption model, Affinity chromatography, Influenza virus, Keywords: Adsorption kinetics, Multi-site binding, Surface plasmon resonance
PMID:
24406143
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24406143
Avidity of influenza virus: Model-based identification of adsorption kinetics from surface plasmon resonance experiments.
Wang W1, Wolff MW2, Reichl U2, Sundmacher K3.
Author information
Abstract
Affinity chromatography and membrane adsorption are highly promising methods for the downstream processing of cell culture-derived influenza virus. For the optimization of this separation process, it is desirable to quantify the kinetics of virus adsorption. For this reason, the adsorption kinetics of the influenza A virus (Puerto Rico/8/34 (H1N1)) on a surface with the immobilized ligand Euronymus europaeus lectin (EEL) was investigated. The adsorption kinetics was experimentally monitored in a microfluidic flow cell by surface plasmon resonance (SPR) spectroscopy. The boundary layer theory was applied to analyze the convective and diffusive mass transport of the virus particles in the SPR flow cell. A multi-site kinetic adsorption model was found to describe the experimentally recorded adsorption curves adequately. According to the proposed model, under the applied experimental conditions, the number of sites (galactose residuals) binding one single virus particle to the EEL surface is in the range of 300 to 460, which is in average about 4% of the total number of sites available on the virus surface. The avidity of individual virus particles to the EEL surface was estimated to be in the order of magnitude of 106M-1s-1.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Adsorption model, Affinity chromatography, Influenza virus, Keywords: Adsorption kinetics, Multi-site binding, Surface plasmon resonance
PMID:
24406143
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24406143