tetano
Editor, Senior Moderator
Austral J Vet Sci
. 2024 May;56(2):85-90.
doi: 10.4206/ajvs.562.01. Epub 2024 May 22. Replication kinetics of novel swine influenza A viruses: an approach to vaccine production
Rodrigo Tapia[SUP] 1 2 [/SUP], Rafael Medina[SUP] 3 4 5 [/SUP], Víctor Neira[SUP] 2 [/SUP]
Affiliations
Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of ≥128 HAU/50 μL with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine-producing laboratories.
Keywords: Influenza A virus; MDCK cells; Vero cells; antigen; swine; vaccine.
. 2024 May;56(2):85-90.
doi: 10.4206/ajvs.562.01. Epub 2024 May 22. Replication kinetics of novel swine influenza A viruses: an approach to vaccine production
Rodrigo Tapia[SUP] 1 2 [/SUP], Rafael Medina[SUP] 3 4 5 [/SUP], Víctor Neira[SUP] 2 [/SUP]
Affiliations
- PMID: 39991269
- PMCID: PMC11845247
- DOI: 10.4206/ajvs.562.01
Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of ≥128 HAU/50 μL with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine-producing laboratories.
Keywords: Influenza A virus; MDCK cells; Vero cells; antigen; swine; vaccine.