tetano
Editor, Senior Moderator
J Virol Methods. 2013 Jul 18. pii: S0166-0934(13)00269-3. doi: 10.1016/j.jviromet.2013.07.015. [Epub ahead of print]
Application of real time RT-PCR for the genetic homogeneity and stability tests of the seed candidates for live attenuated influenza vaccine production.
Shcherbik S, Sergent SB, Davis WG, Shu B, Barnes J, Klimov A, Bousse T.
Source
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333; Battelle, Atlanta, Georgia 30333.
Abstract
Development and improvement of quality control tests for live attenuated vaccines are a high priority because of safety concerns. Live Attenuated Influenza Vaccine (LAIV) viruses are 6:2 reassortants containing the hemagglutinin (HA) and neuroaminidase (NA) gene segments from circulating influenza viruses to induce protective immune responses, and the six internal gene segments from a cold-adapted Master Donor Virus (MDV). LAIV candidate viruses for the 2012-2013 seasons, A/Victoria/361/2011-CDC-LV1 (LV1) and B/Texas/06/2011-CDC-LV2B (LV2B), were created by classical reassortment of A/Victoria/361/2011 and MDV-A A/Leningrad/134/17/57 (H2N2) or B/Texas/06/2011 and MDV-B B/USSR/60/69. In an attempt to provide better identity and stability testing for quality control of vaccine candidates, sensitive real-time RT-PCR assays (rRT-PCR) were developed to detect the presence of undesired gene segments (HA and NA from MDV and the six internal genes from the seasonal influenza viruses). The sensitivity of rRT-PCR assays designed for each gene segment ranged from 0.08 to 0.8 EID50 (50% of Egg Infectious Dose) per reaction for the detection of undesired genes in LV1 and from 0.1 to 1 EID50 per reaction for the detection of undesired genes in LV2B. No undesired genes were detected either before or after five passages of LV1 or LV2B in eggs. The complete genome sequencing of LV1 and LV2B confirmed the results of rRT-PCR, demonstrating the utility of the new rRT-PCR assays to provide the evidence for the homogeneity of the prepared vaccine candidate.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS:
Homogeneity test., Influenza virus, Live vaccine, Real time RT-PCR, Reassortants
PMID:
23872266
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23872266
Application of real time RT-PCR for the genetic homogeneity and stability tests of the seed candidates for live attenuated influenza vaccine production.
Shcherbik S, Sergent SB, Davis WG, Shu B, Barnes J, Klimov A, Bousse T.
Source
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333; Battelle, Atlanta, Georgia 30333.
Abstract
Development and improvement of quality control tests for live attenuated vaccines are a high priority because of safety concerns. Live Attenuated Influenza Vaccine (LAIV) viruses are 6:2 reassortants containing the hemagglutinin (HA) and neuroaminidase (NA) gene segments from circulating influenza viruses to induce protective immune responses, and the six internal gene segments from a cold-adapted Master Donor Virus (MDV). LAIV candidate viruses for the 2012-2013 seasons, A/Victoria/361/2011-CDC-LV1 (LV1) and B/Texas/06/2011-CDC-LV2B (LV2B), were created by classical reassortment of A/Victoria/361/2011 and MDV-A A/Leningrad/134/17/57 (H2N2) or B/Texas/06/2011 and MDV-B B/USSR/60/69. In an attempt to provide better identity and stability testing for quality control of vaccine candidates, sensitive real-time RT-PCR assays (rRT-PCR) were developed to detect the presence of undesired gene segments (HA and NA from MDV and the six internal genes from the seasonal influenza viruses). The sensitivity of rRT-PCR assays designed for each gene segment ranged from 0.08 to 0.8 EID50 (50% of Egg Infectious Dose) per reaction for the detection of undesired genes in LV1 and from 0.1 to 1 EID50 per reaction for the detection of undesired genes in LV2B. No undesired genes were detected either before or after five passages of LV1 or LV2B in eggs. The complete genome sequencing of LV1 and LV2B confirmed the results of rRT-PCR, demonstrating the utility of the new rRT-PCR assays to provide the evidence for the homogeneity of the prepared vaccine candidate.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS:
Homogeneity test., Influenza virus, Live vaccine, Real time RT-PCR, Reassortants
PMID:
23872266
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23872266