tetano
Editor, Senior Moderator
Zhong Xi Yi Jie He Xue Bao. 2010 Nov 15;8(11):1036-1040.
[Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5N1 avian influenza virus.]
[Article in Chinese]
Liu H, Zhang GL, Shen L, Zeng Z, Zhou BL, Liu CH, Nie G.
Department of Integrated Traditional Chinese and Western Medicine, Shenzhen Third People's Hospital, Shenzhen 518020, Guangdong Province, China; E-mail: fqng1008@163.com.
Abstract
Objective: To establish a method based on a pseudotyped virus assay for screening drugs for anti-H5N1 avian influenza virus, and then to evaluate anti-virus activity of traditional Chinese herbal compounds with the function of purgation, detoxification, cooling the blood and reinforcing the healthy qi based on seropharmacology. Methods: Pseudotyped viruses expressing H5N1 virus hemagglutinin were constructed. Herb-medicated sera were prepared by feeding Wistar rats traditional Chinese herbal compounds. Then inhibitory effects of different drug-medicated sera against pseudotyped virus were evaluated based on incubating serum with pseudotyped viruses or target cells such as Madin-Darby canine kidney cells and human embryonic kidney cell line 293T cells, and the expression of hemagglutinin (HA) protein was detected by flow cytometry. Results: Pseudotyped virus could be neutralized by specific 2B4 monoclonal antibody at doses of 50 μL (500μg), 25μL (250μg) and 12.5μL (125μg), which showed that it was a feasible assay to screen drugs. No significant difference was observed in inhibition against H5N1 pseudotyped virus between herb-medicated sera and control serum (P>0.05), meanwhile, the herb-medicated sera also could not inhibit HA expression. Conclusion: Pseudotyped virus assay is a safe, effective, and exact method for screening anti-H5N1 avian influenza virus herbs, and herb-medicated sera prepared by administration of herbs with the function of purgation, detoxification, cooling the blood and reinforcing the healthy qi do not contain active ingredient or monomer which can inhibit HA and sialic acid-α 2,3-galatose.
PMID: 21078267 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21078267?dopt=Abstract
[Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5N1 avian influenza virus.]
[Article in Chinese]
Liu H, Zhang GL, Shen L, Zeng Z, Zhou BL, Liu CH, Nie G.
Department of Integrated Traditional Chinese and Western Medicine, Shenzhen Third People's Hospital, Shenzhen 518020, Guangdong Province, China; E-mail: fqng1008@163.com.
Abstract
Objective: To establish a method based on a pseudotyped virus assay for screening drugs for anti-H5N1 avian influenza virus, and then to evaluate anti-virus activity of traditional Chinese herbal compounds with the function of purgation, detoxification, cooling the blood and reinforcing the healthy qi based on seropharmacology. Methods: Pseudotyped viruses expressing H5N1 virus hemagglutinin were constructed. Herb-medicated sera were prepared by feeding Wistar rats traditional Chinese herbal compounds. Then inhibitory effects of different drug-medicated sera against pseudotyped virus were evaluated based on incubating serum with pseudotyped viruses or target cells such as Madin-Darby canine kidney cells and human embryonic kidney cell line 293T cells, and the expression of hemagglutinin (HA) protein was detected by flow cytometry. Results: Pseudotyped virus could be neutralized by specific 2B4 monoclonal antibody at doses of 50 μL (500μg), 25μL (250μg) and 12.5μL (125μg), which showed that it was a feasible assay to screen drugs. No significant difference was observed in inhibition against H5N1 pseudotyped virus between herb-medicated sera and control serum (P>0.05), meanwhile, the herb-medicated sera also could not inhibit HA expression. Conclusion: Pseudotyped virus assay is a safe, effective, and exact method for screening anti-H5N1 avian influenza virus herbs, and herb-medicated sera prepared by administration of herbs with the function of purgation, detoxification, cooling the blood and reinforcing the healthy qi do not contain active ingredient or monomer which can inhibit HA and sialic acid-α 2,3-galatose.
PMID: 21078267 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21078267?dopt=Abstract