tetano
Editor, Senior Moderator
Antiviral Res
. 2020 Nov 6;104971.
doi: 10.1016/j.antiviral.2020.104971. Online ahead of print.
Structural characterization of the interaction between the C-terminal domain of the influenza polymerase PA subunit and an optimized small peptide inhibitor
Jakub Hejd?nek[SUP] 1 [/SUP], Kateřina Radilov?[SUP] 2 [/SUP], Petr Pachl[SUP] 1 [/SUP], Jan Hodek[SUP] 1 [/SUP], Aleš Machara[SUP] 3 [/SUP], Jan Weber[SUP] 1 [/SUP], Pavl?na Řez?čov?[SUP] 4 [/SUP], Jan Konvalinka[SUP] 5 [/SUP], Milan Kož?šek[SUP] 6 [/SUP]
Affiliations
Abstract
Influenza viruses can cause severe respiratory infections in humans, leading to nearly half a million deaths worldwide each year. Improved antiviral drugs are needed to address the threat of development of novel pandemic strains. Current therapeutic interventions target three key proteins in the viral life cycle: neuraminidase, the M2 channel and RNA-dependent-RNA polymerase. Protein-protein interactions between influenza polymerase subunits are potential new targets for drug development. Using a newly developed assay based on AlphaScreen technology, we screened a peptide panel for protein-protein interaction inhibitors to identify a minimal PB1 subunit-derived peptide that retains high inhibition potential and can be further modified. Here, we present an X-ray structure of the resulting decapeptide bound to the C-terminal domain of PA polymerase subunit from pandemic isolate A/California/07/2009 H1N1 at 1.6 ? resolution and discuss its implications for the design of specific, potent influenza polymerase inhibitors.
Keywords: AlphaScreen; Antiviral peptides; influenza A polymerase; protein-protein interaction.
. 2020 Nov 6;104971.
doi: 10.1016/j.antiviral.2020.104971. Online ahead of print.
Structural characterization of the interaction between the C-terminal domain of the influenza polymerase PA subunit and an optimized small peptide inhibitor
Jakub Hejd?nek[SUP] 1 [/SUP], Kateřina Radilov?[SUP] 2 [/SUP], Petr Pachl[SUP] 1 [/SUP], Jan Hodek[SUP] 1 [/SUP], Aleš Machara[SUP] 3 [/SUP], Jan Weber[SUP] 1 [/SUP], Pavl?na Řez?čov?[SUP] 4 [/SUP], Jan Konvalinka[SUP] 5 [/SUP], Milan Kož?šek[SUP] 6 [/SUP]
Affiliations
- PMID: 33166574
- DOI: 10.1016/j.antiviral.2020.104971
Abstract
Influenza viruses can cause severe respiratory infections in humans, leading to nearly half a million deaths worldwide each year. Improved antiviral drugs are needed to address the threat of development of novel pandemic strains. Current therapeutic interventions target three key proteins in the viral life cycle: neuraminidase, the M2 channel and RNA-dependent-RNA polymerase. Protein-protein interactions between influenza polymerase subunits are potential new targets for drug development. Using a newly developed assay based on AlphaScreen technology, we screened a peptide panel for protein-protein interaction inhibitors to identify a minimal PB1 subunit-derived peptide that retains high inhibition potential and can be further modified. Here, we present an X-ray structure of the resulting decapeptide bound to the C-terminal domain of PA polymerase subunit from pandemic isolate A/California/07/2009 H1N1 at 1.6 ? resolution and discuss its implications for the design of specific, potent influenza polymerase inhibitors.
Keywords: AlphaScreen; Antiviral peptides; influenza A polymerase; protein-protein interaction.