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Antiviral Res. Amantadine-resistant influenza A viruses isolated in South Korea from 2003 to 2009.

Giuseppe

Emeritus
Antiviral Res. 2009 Aug 28. [Epub ahead of print]

Amantadine-resistant influenza A viruses isolated in South Korea from 2003 to 2009.

Choi WY, Kim S, Lee N, Kwon M, Yang I, Kim MJ, Cheong SG, Kwon D, Lee JY, Oh HB, Kang C. - Division of Influenza and Respiratory Viruses, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control & Prevention, Seoul, Korea.

To investigate the frequency of amatadine resistance among influenza A viruses isolated in Korea during the 2003-2009 seasons, 369 (16.8%) 2,199 A/H1N1 viruses and 780 (14.8%) of 5,263 A/H3N2 viruses were randomly selected. The M2 and HA1 genes of each isolate were amplified by reverse transcription-polymerase chain reaction and followed by nucleotide sequencing. The results showed that the resistance rate to amantadine among A/H1N1 viruses increased significantly from 2004-2005 (33.3%) to 2007-2008 (97.8%) and then decreased dramatically in 2008-2009 (1.9%). The A/H1N1 isolates recently detected in 2008-2009 turned amantadine-sensitive containing two new substitutions at specific sites (S141N, G185A) in HA1. Compared with A/H1N1 viruses, the amantadine resistance among the A/H3N2 viruses increased from 2003-2004 (9.7%) to 2005-2006 (96.7%) and decreased in 2006-2007 (57.4%). During 2006-2007, both of amantadine-resistant and -sensitive A/H3N2 viruses co-circulated but clustered in different branches phylogenetically. All of A/H3N2 isolates tested during 2007-2009 appeared to cluster in the same group being resistant to amantadine.

PMID: 19720085 [PubMed - as supplied by publisher]

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