Giuseppe
Emeritus
[Source: Antimicrobial Agents and Chemotherapy, full text: (LINK). Abstract, edited.]
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Wide dissemination of GES-type carbapenemases in Acinetobacter baumannii in Kuwait
R?my A. Bonnin 1, Vincent O. Rotimi 2, Mona Al Hubail 2, Elise Gasiorowski 1, Noura Al Sweih 2, Patrice Nordmann 1↴ and Laurent Poirel 1
Author Affiliations: <SUP>1</SUP>Service de Bact?riologie-Virologie, INSERM U914 ? Emerging Resistance to Antibiotics ?, H?pital de Bic?tre, Assistance Publique/H?pitaux de Paris, Facult? de M?decine et Universit? Paris-Sud, K.-Bic?tre, France; <SUP>2</SUP>Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait
ABSTRACT
Acinetobacter baumannii is an opportunistic pathogen that is an important source of nosocomial infections. Production of extended-spectrum β-lactamases (ESBLs) of the GES-type are reported increasingly in A. baumannii, and some of these GES-type enzymes possess some carbapenemase activity. Our aim was to analyze the resistance determinants and the clonal relationship of carbapenem non-susceptible A. baumannii clinical isolates recovered from hospitals in Kuwait. A total of sixty-three isolates were analyzed and all were found positive for bla<SUB>GES</SUB>-type genes. One isolate harbored the bla<SUB>GES-14</SUB> gene encoding an ESBL with significant carbapenemase activity, whereas the other isolates harbored the bla<SUB>GES-11</SUB> ESBL gene. Thirty-three isolates co-harbored the bla<SUB>OXA-23</SUB> and bla<SUB>GES-11</SUB> genes. The analysis of the genetic location indicated that the bla<SUB>GES-11/-14</SUB> genes were plasmid-located. Noteworthy, the bla<SUB>OXA-23</SUB> and bla<SUB>GES-11</SUB> genes were co-located onto a single plasmid belonging. Nine different pulsotypes were observed among the sixty-three isolates. This study showed the emergence of GES-type ESBLs in A. baumannii in Kuwait, further suggesting that the Middle East region might be a reservoir for carbapenemase-producing A. baumannii.
FOOTNOTES
Corresponding author. Mailing address: Service de Bact?riologie-Virologie-Hygi?ne, H?pital de Bic?tre, 78 rue de G?n?ral Leclerc, 94275 Le Kremlin-Bic?tre Cedex, France., Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40, E-mail: nordmann.patrice@bct.aphp.fr
Copyright ? 2012, American Society for Microbiology. All Rights Reserved.
-Author Affiliations: <SUP>1</SUP>Service de Bact?riologie-Virologie, INSERM U914 ? Emerging Resistance to Antibiotics ?, H?pital de Bic?tre, Assistance Publique/H?pitaux de Paris, Facult? de M?decine et Universit? Paris-Sud, K.-Bic?tre, France; <SUP>2</SUP>Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait
ABSTRACT
Acinetobacter baumannii is an opportunistic pathogen that is an important source of nosocomial infections. Production of extended-spectrum β-lactamases (ESBLs) of the GES-type are reported increasingly in A. baumannii, and some of these GES-type enzymes possess some carbapenemase activity. Our aim was to analyze the resistance determinants and the clonal relationship of carbapenem non-susceptible A. baumannii clinical isolates recovered from hospitals in Kuwait. A total of sixty-three isolates were analyzed and all were found positive for bla<SUB>GES</SUB>-type genes. One isolate harbored the bla<SUB>GES-14</SUB> gene encoding an ESBL with significant carbapenemase activity, whereas the other isolates harbored the bla<SUB>GES-11</SUB> ESBL gene. Thirty-three isolates co-harbored the bla<SUB>OXA-23</SUB> and bla<SUB>GES-11</SUB> genes. The analysis of the genetic location indicated that the bla<SUB>GES-11/-14</SUB> genes were plasmid-located. Noteworthy, the bla<SUB>OXA-23</SUB> and bla<SUB>GES-11</SUB> genes were co-located onto a single plasmid belonging. Nine different pulsotypes were observed among the sixty-three isolates. This study showed the emergence of GES-type ESBLs in A. baumannii in Kuwait, further suggesting that the Middle East region might be a reservoir for carbapenemase-producing A. baumannii.
FOOTNOTES
Corresponding author. Mailing address: Service de Bact?riologie-Virologie-Hygi?ne, H?pital de Bic?tre, 78 rue de G?n?ral Leclerc, 94275 Le Kremlin-Bic?tre Cedex, France., Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40, E-mail: nordmann.patrice@bct.aphp.fr
Copyright ? 2012, American Society for Microbiology. All Rights Reserved.
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