Giuseppe
Emeritus
[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]
Prevalence and molecular characterization of fluoroquinolone-resistant Mycobacterium tuberculosis isolates in China
Zhijian Zhang 1,2, Jie Lu 3, Yufeng Wang 2, Yu Pang 1,2* and Yanlin Zhao 2,2*
Author Affiliations: Respiratory Diseases Department of Nanlou, Chinese People's Liberation Army General Hospital<SUP>1 </SUP>National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China<SUP>2 </SUP>Beijing Pediatric Research Institute, Beijing Children's Hospital affiliated to Capital Medical University, Beijing, China<SUP>3</SUP>
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ABSTRACT
China is one of the countries with the highest multidrug-resistant (MDR) and fluroquinolone (FQ)-resistant TB burden country globally. Nevertheless, knowledge on the prevalence and molecular characterization of FQ-resistantMycobacterium tuberculosis (M. tuberculosis) isolates from this region remains not numerous. In this study, by utilizing agar proportion susceptibility method, the 138 ofloxacin (OFX)-resistant M. tuberculosis isolates were enrolled from the national drug resistance survey of China. All these M. tuberculosis strains were tested for drug susceptibility to ofloxacin, levofloxacin, moxifloxacin, gatifloxacin and sparfloxacin using the liquid Middlebrook 7H9 medium. The entire gyrA and gyrB genes conferring FQ resistance were sequenced, and the spoligotyping was performed to distinguish different genotypes. Overall, the prevalence of FQ resistance in China was highest for ofloxacin (3.76%), intermediate for levofloxacin (3.18%) and moxifloxacin (3.12%), and lowest for sparfloxacin (1.91%) and gatifloxacin (1.33%). Mutations in gyrA gene were observed in 89 (64.5%) out of 138 OFX-resistant M. tuberculosis strains. Position 94 and 90 was the most frequent mutation site conferring FQ resistance in these strains, accounting for high-level FQ resistance. Furthermore, Beijing genotype showed no association with high-level FQ resistance and distribution in hot spots in QRDR of gyrA. Our findings will provide essential implications into the feasibility of genotypic tests relying on detecting mutations in the QRDR of gyrAand the shorter first-line treatment regimens based on FQs in China.
FOOTNOTES
*Corresponding author. Mailing address for Yu Pang: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Chang Bai Road, Changping District, Beijing 102206, People's Republic of China. Phone: 86 10 5890 0779. Fax: 86 10 5890 0779. E-mail: pangyu@chinatb.org. Mailing address for Yanlin Zhao: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Chang Bai Road, Changping District, Beijing 102206, People's Republic of China. Phone: 86 10 5890 0777. Fax: 86 10 5890 0779. E-mail: zhaoyanlin@tb123.org.
Copyright ? 2013, American Society for Microbiology. All Rights Reserved.
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Prevalence and molecular characterization of fluoroquinolone-resistant Mycobacterium tuberculosis isolates in China
Zhijian Zhang 1,2, Jie Lu 3, Yufeng Wang 2, Yu Pang 1,2* and Yanlin Zhao 2,2*
Author Affiliations: Respiratory Diseases Department of Nanlou, Chinese People's Liberation Army General Hospital<SUP>1 </SUP>National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China<SUP>2 </SUP>Beijing Pediatric Research Institute, Beijing Children's Hospital affiliated to Capital Medical University, Beijing, China<SUP>3</SUP>
<SUP></SUP>
ABSTRACT
China is one of the countries with the highest multidrug-resistant (MDR) and fluroquinolone (FQ)-resistant TB burden country globally. Nevertheless, knowledge on the prevalence and molecular characterization of FQ-resistantMycobacterium tuberculosis (M. tuberculosis) isolates from this region remains not numerous. In this study, by utilizing agar proportion susceptibility method, the 138 ofloxacin (OFX)-resistant M. tuberculosis isolates were enrolled from the national drug resistance survey of China. All these M. tuberculosis strains were tested for drug susceptibility to ofloxacin, levofloxacin, moxifloxacin, gatifloxacin and sparfloxacin using the liquid Middlebrook 7H9 medium. The entire gyrA and gyrB genes conferring FQ resistance were sequenced, and the spoligotyping was performed to distinguish different genotypes. Overall, the prevalence of FQ resistance in China was highest for ofloxacin (3.76%), intermediate for levofloxacin (3.18%) and moxifloxacin (3.12%), and lowest for sparfloxacin (1.91%) and gatifloxacin (1.33%). Mutations in gyrA gene were observed in 89 (64.5%) out of 138 OFX-resistant M. tuberculosis strains. Position 94 and 90 was the most frequent mutation site conferring FQ resistance in these strains, accounting for high-level FQ resistance. Furthermore, Beijing genotype showed no association with high-level FQ resistance and distribution in hot spots in QRDR of gyrA. Our findings will provide essential implications into the feasibility of genotypic tests relying on detecting mutations in the QRDR of gyrAand the shorter first-line treatment regimens based on FQs in China.
FOOTNOTES
*Corresponding author. Mailing address for Yu Pang: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Chang Bai Road, Changping District, Beijing 102206, People's Republic of China. Phone: 86 10 5890 0779. Fax: 86 10 5890 0779. E-mail: pangyu@chinatb.org. Mailing address for Yanlin Zhao: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Chang Bai Road, Changping District, Beijing 102206, People's Republic of China. Phone: 86 10 5890 0777. Fax: 86 10 5890 0779. E-mail: zhaoyanlin@tb123.org.
Copyright ? 2013, American Society for Microbiology. All Rights Reserved.
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